ArticleScientific reports2017
Graphene oxide enhances the specificity of the polymerase chain reaction by modifying primer-template matching.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Nanomaterials in PCR: exploring light-to-heat conversion mechanisms and microfluidic integration.Microsystems & nanoengineering · 2025Review
- Nanoparticle-assisted PCR: fundamentals, mechanisms, and forensic implications.International journal of legal medicine · 2025Review
- Single-layer graphene oxide film grown on α-AlBeilstein journal of nanotechnology · 2025Article
- Enhancement Effects and Mechanism Studies of Two Bismuth-Based Materials Assisted by DMSO and Glycerol in GC-Rich PCR.Molecules (Basel, Switzerland) · 2023Article
- Development and preliminary assessment of a CRISPR-Cas12a-based multiplex detection ofFrontiers in bioengineering and biotechnology · 2023Article
- Application of Nanomaterials to Enhance Polymerase Chain Reaction.Molecules (Basel, Switzerland) · 2022Review
- High sensitivity SARS-CoV-2 detection using graphene oxide-multiplex qPCR.Analytica chimica acta · 2022Article
- Article
- Multifunctional carbon nanomaterials for diagnostic applications in infectious diseases and tumors.Materials today. Bio · 2022Review
- Graphene oxide and self-avoiding molecular recognition systems-assisted recombinase polymerase amplification coupled with lateral flow bioassay for nucleic acid detection.Mikrochimica acta · 2020Article
- Effects of graphene oxide on PCR amplification for microbial community survey.BMC microbiology · 2020Article
- Enhancement of Gas Barrier Properties of Graphene Oxide/Poly (Lactic Acid) Films Using a Solvent-free Method.Materials (Basel, Switzerland) · 2020Article
- Graphene Oxide Nano-Concentrators Selectively Modulate RNA Trapping According to Metal Cations in Solution.Frontiers in bioengineering and biotechnology · 2020Article
- Thermally stable and uniform DNA amplification with picosecond laser ablated graphene rapid thermal cycling device.Biosensors & bioelectronics · 2019Article
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Authors and funding
4 authors.
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Abstract
Aiming at improved specificity, nanoparticle assisted polymerase chain reaction (PCR) has been widely studied and shown to improve PCR. However, the reliability and mechanism of this method are still controversial. Here, we demonstrated that 1 μg/mL of graphene oxide (GO) effectively enhances the specificity of the error-prone multi-round PCR. Mismatched primers were designed as interference to produce nonspecific products when the same amounts of matched and mismatched primers were added into semi-multiplex PCR. It was found that GO can enhance specificity by suppressing the amplification of mismatched primers. We monitored the primer-template-polymerase-GO interactions involved in the PCR using a capillary electrophoresis/laser-induced fluorescence polarization (CE-LIFP) assay. The results showed that the addition of GO promoted the formation of a matched primer-template complex, but suppressed the formation of a mismatched primer-template complex during PCR, suggesting that interactions between the primers and GO play an essential role. Furthermore, we successfully amplified the FOXL2 gene from PEGFP-N1 vectors using GO to eliminate the nonspecific products in PCR. Taken together, these results suggest that the GO can be used as an efficient additive for improving the conventional PCR system.
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