ArticleNature neuroscience2017
Temporally precise single-cell-resolution optogenetics.
Article in Nature neuroscience, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 150 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
150 citing papers in PubMed.
- Photomapping electrically coupled networks of the mature thalamus and cortex.Science advances · 2026Article
- CLOK: a chemigenetic multicolor labeling system to visualize neuronal birthdate and circuit integration.Nature neuroscience · 2026Article
- Voltage imaging of neurons distributed across entire brains of larval zebrafish.Nature methods · 2026Article
- Crosstalk-free optical indicator-actuator combinations for bidirectional optogenetics.npj biomedical innovations · 2026Review
- Neuronal ensembles in cortical function and disease.Physiological reviews · 2026Review
- The Importance of Synchrony in the Neural Control of Movement.bioRxiv : the preprint server for biology · 2026Article
- Postnatal Development of Pyramidal Neurons Excitability and Synaptic Inputs in Mouse Gustatory Cortical Circuits.eNeuro · 2026Article
- Computational optimization of two-photon holographic stimulation sitesJournal of neural engineering · 2026Article
- Photomapping electrically coupled networks of the mature thalamus and cortex.bioRxiv : the preprint server for biology · 2026Article
- A Closed-Loop-Capable Neural Interface Platform for Deep Brain Modulation via Integrated Non-Viral Gene Delivery, NIR Optogenetics, and Electrophysiological Recording.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Very-large-scale mimetic optogenetic synapses for physical reservoir computing.Nature communications · 2026Article
- Genetic manipulation technologies for precise cellular control: a comparative review of five approaches.Frontiers in bioengineering and biotechnology · 2026Review
- Laser-engineered PRIME fiber for panoramic reconfigurable control of neural activity.Nature neuroscience · 2026Article
- Closed-loop optogenetic control of cell biology enables outcome-driven microscopy.Nature communications · 2025Article
- Optogenetic interrogation of the zebrafish lateral line reveals brain-wide neural circuits involved in pattern separation.iScience · 2025Article
- Red-shifted excitation enhances the sensitivity of red genetically encoded CaJournal of biomedical optics · 2025Article
- Rapid learning of neural circuitry from holographic ensemble stimulation enabled by model-based compressed sensing.Nature neuroscience · 2025Article
- High-throughput synaptic connectivity mapping using in vivo two-photon holographic optogenetics and compressive sensing.Nature neuroscience · 2025Article
- Monolithic multimodal neural probes for sustained stimulation and long-term neural recording.Science advances · 2025Article
- Exact linear theory of perturbation response in a space- and feature-dependent cortical circuit model.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
90 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
9 authors.
Funding
Abstract
Optogenetic control of individual neurons with high temporal precision within intact mammalian brain circuitry would enable powerful explorations of how neural circuits operate. Two-photon computer-generated holography enables precise sculpting of light and could in principle enable simultaneous illumination of many neurons in a network, with the requisite temporal precision to simulate accurate neural codes. We designed a high-efficacy soma-targeted opsin, finding that fusing the N-terminal 150 residues of kainate receptor subunit 2 (KA2) to the recently discovered high-photocurrent channelrhodopsin CoChR restricted expression of this opsin primarily to the cell body of mammalian cortical neurons. In combination with two-photon holographic stimulation, we found that this somatic CoChR (soCoChR) enabled photostimulation of individual cells in mouse cortical brain slices with single-cell resolution and <1-ms temporal precision. We used soCoChR to perform connectivity mapping on intact cortical circuits.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.