ArticleDevelopmental biology2018
Cross-platform single cell analysis of kidney development shows stromal cells express Gdnf.
Article in Developmental biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 98 citations in OpenAlex.
- Kidney organoids as models for hereditary kidney diseases: Toward precision medicine (Review).Experimental and therapeutic medicine · 2026Review
- Synthetic budding morphogenesis by optogenetic receptor tyrosine kinase signaling.bioRxiv : the preprint server for biology · 2026Article
- The transcription factor Tcf21 is necessary for adoption of cell fates byAmerican journal of physiology. Renal physiology · 2026Article
- Podocyte involvement in the pathogenesis of preterm-related long-term chronic kidney disease.Histology and histopathology · 2024Review
- Nephron progenitors rhythmically alternate between renewal and differentiation phases that synchronize with kidney branching morphogenesis.bioRxiv : the preprint server for biology · 2024Article
- Vascular endothelial cell development and diversity.Nature reviews. Cardiology · 2023Review
- Hedgehog-GLI mediated control of renal formation and malformation.Frontiers in nephrology · 2023Review
- Characterization of alternative mRNA splicing in cultured cell populations representing progressive stages of human fetal kidney development.Scientific reports · 2022Article
- Regulation of nephron progenitor cell lifespan and nephron endowment.Nature reviews. Nephrology · 2022Review
- Mapping the human kidney using single-cell genomics.Nature reviews. Nephrology · 2022Review
- Comparative whole-genome transcriptome analysis in renal cell populations reveals high tissue specificity of MAPK/ERK targets in embryonic kidney.BMC biology · 2022Article
- Assessing kidney development and disease using kidney organoids and CRISPR engineering.Frontiers in cell and developmental biology · 2022Review
- Determining lineage relationships in kidney development and disease.Nature reviews. Nephrology · 2022Review
- Drug-Induced Nephrotoxicity Assessment in 3D Cellular Models.Micromachines · 2021Review
- The study of single cells in diabetic kidney disease.Journal of nephrology · 2021Review
- Single-cell RNA sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis.Scientific reports · 2021Article
- Progenitor translatome changes coordinated by Tsc1 increase perception of Wnt signals to end nephrogenesis.Nature communications · 2021Article
- Microfluidics applications for high-throughput single cell sequencing.Journal of nanobiotechnology · 2021Review
- The origin and role of the renal stroma.Development (Cambridge, England) · 2021Review
- Single cell sequencing technology and its application in Hypoxic ischemic encephalopathy research.Ibrain · 2021Review
1 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
The developing kidney provides a useful model for study of the principles of organogenesis. In this report we use three independent platforms, Drop-Seq, Chromium 10x Genomics and Fluidigm C1, to carry out single cell RNA-Seq (scRNA-Seq) analysis of the E14.5 mouse kidney. Using the software AltAnalyze, in conjunction with the unsupervised approach ICGS, we were unable to identify and confirm the presence of 16 distinct cell populations during this stage of active nephrogenesis. Using a novel integrative supervised computational strategy, we were able to successfully harmonize and compare the cell profiles across all three technological platforms. Analysis of possible cross compartment receptor/ligand interactions identified the nephrogenic zone stroma as a source of GDNF. This was unexpected because the cap mesenchyme nephron progenitors had been thought to be the sole source of GDNF, which is a key driver of branching morphogenesis of the collecting duct system. The expression of Gdnf by stromal cells was validated in several ways, including Gdnf in situ hybridization combined with immunohistochemistry for SIX2, and marker of nephron progenitors, and MEIS1, a marker of stromal cells. Finally, the single cell gene expression profiles generated in this study confirmed and extended previous work showing the presence of multilineage priming during kidney development. Nephron progenitors showed stochastic expression of genes associated with multiple potential differentiation lineages.
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