ArticleBMC research notes2017
Optimization of PCR conditions for amplifying an AT-rich amino acid transporter promoter sequence with high number of tandem repeats from Arabidopsis thaliana.
Article in BMC research notes, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Complete Mitochondrial Genome ofGenes · 2026Article
- Evolution, structure and function of L-cysteine desulfidase, an enzyme involved in sulfur metabolism in the methanogenic archeon Methanococcus maripaludis.Communications biology · 2025Article
- Optimizing PCR amplification of GC-rich nicotinic acetylcholine receptor subunits from invertebrates.Biochemistry and biophysics reports · 2025Article
- A new method to synthesize multiple gRNA libraries and functional mapping of mammalian H3K4me3 regions.Nucleic acids research · 2023Article
- R3-MYB repressor Mybr97 is a candidate gene associated with the Anthocyanin3 locus and enhanced anthocyanin accumulation in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Effect of ACGT motif in spatiotemporal regulation of AtAVT6D, which improves tolerance to osmotic stress and nitrogen-starvation.Plant molecular biology · 2022Article
- Low guanine content and biased nucleotide distribution in vertebrate mtDNA can cause overestimation of non-CpG methylation.NAR genomics and bioinformatics · 2022Article
- Avian Malaria in Penguins: Diagnostics and Future Direction in the Context of Climate Change.Animals : an open access journal from MDPI · 2022Review
- A CRISPR-Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection.Nature communications · 2018Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
objectiveThe aim of the present study is to optimize the PCR conditions required to amplify the promoter sequence of an amino acid transporter having an AT-rich base composition with a high number of tandem repeats.
resultResults show that successful amplification can be achieved by performing a 2-step PCR at a lower extension temperature of 65 °C for an increased extension period of 1.5 min/kb, with MgCl
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Registered trials
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