Evidence map›Paper›PMID 29183316›Full record

ArticleBMC veterinary research2017

RNA sequencing reveals candidate genes and polymorphisms related to sperm DNA integrity in testis tissue from boars.

Maren van Son, Nina Hårdnes Tremoen, Ann Helen Gaustad, Frøydis Deinboll Myromslien, Dag Inge Våge, Else-Berit Stenseth, Teklu Tewoldebrhan Zeremichael, Eli Grindflek

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
3.6field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 29 citations in OpenAlex.

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  4. Review
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  10. Progress in Research on Sperm DNA Fragmentation.Medical science monitor : international medical journal of experimental and clinical research · 2020
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Maren van SonTopigs Norsvin, 2317, Hamar, Norway. maren.van.son@norsvin.no.ORCID http://orcid.org/0000-0002-4892-3387
Nina Hårdnes TremoenDepartment of Natural Sciences and Technology, Inland Norway University of Applied Sciences, 2318, Hamar, Norway.
Ann Helen GaustadTopigs Norsvin, 2317, Hamar, Norway.
Frøydis Deinboll MyromslienDepartment of Natural Sciences and Technology, Inland Norway University of Applied Sciences, 2318, Hamar, Norway.
Dag Inge VågeCentre for Integrative Genetics (CIGENE), Department of Animal and Aquacultural Sciences, Faculty of Biosciences, Norwegian University of Life Sciences, 1432, Ås, Norway.
Else-Berit StensethDepartment of Natural Sciences and Technology, Inland Norway University of Applied Sciences, 2318, Hamar, Norway.
Teklu Tewoldebrhan ZeremichaelDepartment of Natural Sciences and Technology, Inland Norway University of Applied Sciences, 2318, Hamar, Norway.
Eli GrindflekTopigs Norsvin, 2317, Hamar, Norway.
University of Inland Norway · NONorsvin (Norway) · NONorwegian University of Life Sciences · NO

Funding

Norges Forskningsråd 207568
6 · The paper itself

Abstract

backgroundSperm DNA is protected against fragmentation by a high degree of chromatin packaging. It has been demonstrated that proper chromatin packaging is important for boar fertility outcome. However, little is known about the molecular mechanisms underlying differences in sperm DNA fragmentation. Knowledge of sequence variation influencing this sperm parameter could be beneficial in selecting the best artificial insemination (AI) boars for commercial production. The aim of this study was to identify genes differentially expressed in testis tissue of Norwegian Landrace and Duroc boars, with high and low sperm DNA fragmentation index (DFI), using transcriptome sequencing.

resultsAltogether, 308 and 374 genes were found to display significant differences in expression level between high and low DFI in Landrace and Duroc boars, respectively. Of these genes, 71 were differentially expressed in both breeds. Gene ontology analysis revealed that significant terms in common for the two breeds included extracellular matrix, extracellular region and calcium ion binding. Moreover, different metabolic processes were enriched in Landrace and Duroc, whereas immune response terms were common in Landrace only. Variant detection identified putative polymorphisms in some of the differentially expressed genes. Validation showed that predicted high impact variants in RAMP2, GIMAP6 and three uncharacterized genes are particularly interesting for sperm DNA fragmentation in boars.

conclusionsWe identified differentially expressed genes between groups of boars with high and low sperm DFI, and functional annotation of these genes point towards important biochemical pathways. Moreover, variant detection identified putative polymorphisms in the differentially expressed genes. Our results provide valuable insights into the molecular network underlying DFI in pigs.

Indexed as

DNA FragmentationGene Expression ProfilingAnimalsBreedingMalePolymorphism, GeneticSequence Analysis, RNASpermatozoaSus scrofaTestisTranscriptomeDifferential expressionSperm DNA integrityTranscriptome profiling

Identifiers

PMID29183316
PMCPMC5706377
OpenAlexW2770952578

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.