ArticleAnalytical chemistry2017
Remote Activation of a Nanopore for High-Performance Genetic Detection Using a pH Taxis-Mimicking Mechanism.
Article in Analytical chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Controlling DNA Fragments Translocation across Nanopores with the Synergic Use of Site-Directed Mutagenesis, pH-Dependent Charge Tuning, and Electroosmotic Flow.ACS applied materials & interfaces · 2024Article
- Nanopore-based disease diagnosis using pathogen-derived tryptic peptides from serum.Nano today · 2022Article
- Biological nanopores for single-molecule sensing.iScience · 2022Review
- An engineered third electrostatic constriction of aerolysin to manipulate heterogeneously charged peptide transport.Chemical science · 2022Article
- Recent advances in biological nanopores for nanopore sequencing, sensing and comparison of functional variations in MspA mutants.RSC advances · 2021Review
- Insight into the effects of electrochemical factors on host-guest interaction induced signature events in a biological nanopore.Nanotechnology and Precision Engineering · 2020Article
- Revealing the transient conformations of a single flavin adenine dinucleotide using an aerolysin nanopore.Chemical science · 2019Article
- A lithium-ion-active aerolysin nanopore for effectively trapping long single-stranded DNA.Chemical science · 2019Article
- Single Molecule Study of Hydrogen Bond Interactions Between Single Oligonucleotide and Aerolysin Sensing Interface.Frontiers in chemistry · 2019Article
- The aerolysin nanopore: from peptidomic to genomic applications.Nanoscale · 2018Review
- Mapping the sensing spots of aerolysin for single oligonucleotides analysis.Nature communications · 2018Article
- Membrane protein-based biosensors.Journal of the Royal Society, Interface · 2018Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Aerolysin protein pore has been widely used for sensing peptides and proteins. However, only a few groups explored this nanopore for nucleic acids detection. The challenge is the extremely low capture efficiency for nucleic acids (>10 bases), which severely lowers the sensitivity of an aerolysin-based genetic biosensor. Here we reported a simple and easy-to-operate approach to noncovalently transform aerolysin into a highly nucleic acids-sensitive nanopore. Through a remote pH-modulation mechanism, we simply lower the pH on one side of the pore, then aerolysin is immediately "activated" and enabled to capture target DNA/RNA efficiently from the opposite side of the pore. This mechanism also decelerates DNA translocation, a desired property for sequencing and gene detection, allowing temporal separation of DNAs in different lengths. This method provides insight into the nanopore engineering for biosensing, making aerolysin applicable in genetic and epigenetic detections of long nucleic acids.
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Registered trials
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