ArticlePloS one2017
Arabidopsis ubiquitin ligase PUB12 interacts with and negatively regulates Chitin Elicitor Receptor Kinase 1 (CERK1).
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- Crosstalk and reciprocal regulation of phosphorylation and ubiquitination mediated by receptor-like kinases and U-box E3 ligases during plant growth and environmental stress responses.Plant communications · 2026Review
- Microfluidic Interrogation of Chitin-Induced Calcium Oscillations in the MossPlants (Basel, Switzerland) · 2026Article
- Ubiquitin-Mediated Proteolysis as a Regulator of the Plant Defense-Growth Balance.Plants (Basel, Switzerland) · 2026Review
- Oligosaccharide elicitors in plant immunity: Molecular mechanisms and disease resistance strategies.Plant communications · 2025Review
- Museum Genomics Reveals Temporal Genetic Stasis and Global Genetic Diversity in Arabidopsis thaliana.Molecular ecology · 2025Article
- Attenuation of plant immune kinases by U-box type E3 ubiquitin ligases.Science China. Life sciences · 2025Article
- Ubiquitylation of PHYTOSULFOKINE RECEPTOR 1 modulates the defense response in tomato.Plant physiology · 2023Article
- Paradigms of receptor kinase signaling in plants.The Biochemical journal · 2023Review
- Enhanced Resistance to Fungal and Bacterial Diseases Due to Overexpression of BSR1, a Rice RLCK, in Sugarcane, Tomato, and Torenia.International journal of molecular sciences · 2023Article
- Transcriptomic analysis of pea plant responses to chitooligosaccharides' treatment revealed stimulation of mitogen-activated protein kinase cascade.Frontiers in plant science · 2023Article
- Cooperative regulation of PBI1 and MAPKs controls WRKY45 transcription factor in rice immunity.Nature communications · 2022Article
- Ubiquitination of Receptorsomes, Frontline of Plant Immunity.International journal of molecular sciences · 2022Review
- Interactome of Arabidopsis Thaliana.Plants (Basel, Switzerland) · 2022Article
- A bZIP transcription factor, PqbZIP1, is involved in the plant defense response of American ginseng.PeerJ · 2022Article
- OsPUB41, a U-box E3 ubiquitin ligase, acts as a negative regulator of drought stress response in rice (Oryza Sativa L.).Plant molecular biology · 2021Article
- The maize ZmMIEL1 E3 ligase and ZmMYB83 transcription factor proteins interact and regulate the hypersensitive defence response.Molecular plant pathology · 2021Article
- Lysin Motif (LysM) Proteins: Interlinking Manipulation of Plant Immunity and Fungi.International journal of molecular sciences · 2021Review
- The ubiquitin system affects agronomic plant traits.The Journal of biological chemistry · 2020Review
- Crosstalk between Ubiquitination and Other Post-translational Protein Modifications in Plant Immunity.Plant communications · 2020Review
- Activation of Early Defense Signals in Seedlings ofPlants (Basel, Switzerland) · 2020Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Arabidopsis, fungal chitin is recognized as a pathogen-associated molecular pattern (PAMP) by the chitin receptor complex containing the lysin-motif (LysM) receptor-like kinases CERK1 and LYK5. Upon the perception of chitin, CERK1 phosphorylates the receptor-like cytoplasmic kinase, PBL27, which activates the intracellular mitogen-activated protein kinase (MAPK) cascade. However, the mechanisms by which the CERK1-PBL27 complex is regulated remain largely unknown. We identified ubiquitin ligase PUB12 as a component of the PBL27 complex using co-immunoprecipitation and mass spectrometry. However, PUB12 did not interact directly with PBL27. Instead, the ARM domains of PUB12 and its paralog PUB13 interacted with the intracellular domain of CERK1 in a manner that was dependent on its autophosphorylation, suggesting that the phosphorylation-based auto-activation of CERK1 may be required for its interaction with PUB12. The co-expression of PUB12 in Nicotiana benthamiana reduced the accumulation of CERK1. The pub12 pub13 mutant exhibited enhanced chitin-induced immune responses such as ROS production, MAPK activation, and callose deposition. These results suggest that PUB12 and PUB13 are involved in the negative regulation of the chitin receptor complex, which may contribute to the transient desensitization of chitin-induced responses.
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