Evidence map›Paper›PMID 29182568›Full record

ArticleSensors (Basel, Switzerland)2017

A Quartz Crystal Microbalance Immunosensor for Stem Cell Selection and Extraction.

Ornella Maglio, Salvatore Costanzo, Rosaria Cercola, Gerardo Zambrano, Marco Mauro, Raffaele Battaglia, Gianluca Ferrini, Flavia Nastri, Vincenzo Pavone, Angela Lombardi

Open access · goldAbstract read
In one paragraph

Article in Sensors (Basel, Switzerland), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 30 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Artificial Heme Enzymes for the Construction of Gold-Based Biomaterials.International journal of molecular sciences · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Ornella MaglioDepartment of Chemical Sciences, University of Napoli "Federico II" Via Cintia, 80126 Napoli, Italy. ornella.maglio@unina.it.ORCID 0000-0002-7829-1907
Salvatore CostanzoDepartment of Chemical Sciences, University of Napoli "Federico II" Via Cintia, 80126 Napoli, Italy. salvatore.costanzo@upmc.fr.
Rosaria CercolaDepartment of Chemical Sciences, University of Napoli "Federico II" Via Cintia, 80126 Napoli, Italy. rc1274@york.ac.uk.ORCID 0000-0002-2219-6353
Gerardo ZambranoDepartment of Chemical Sciences, University of Napoli "Federico II" Via Cintia, 80126 Napoli, Italy. gerardo.zambrano@unina.it.
Marco MauroNovaetech S.r.l., Centro Direzionale, Isola G7, 80143 Napoli, Italy. mauro@novaetech.com.
Raffaele BattagliaNovaetech S.r.l., Centro Direzionale, Isola G7, 80143 Napoli, Italy. battaglia@novaetech.com.
Gianluca FerriniNovaetech S.r.l., Centro Direzionale, Isola G7, 80143 Napoli, Italy. ferrini@novaetech.com.
Flavia NastriDepartment of Chemical Sciences, University of Napoli "Federico II" Via Cintia, 80126 Napoli, Italy. flavia.nastri@unina.it.
Vincenzo PavoneDepartment of Chemical Sciences, University of Napoli "Federico II" Via Cintia, 80126 Napoli, Italy. vincenzo.pavone@unina.it.
Angela LombardiDepartment of Chemical Sciences, University of Napoli "Federico II" Via Cintia, 80126 Napoli, Italy. alombard@unina.it.ORCID 0000-0002-2013-3009
University of Naples Federico II · ITConsorzio Catania Ricerche · ITInstitute of Biostructure and Bioimaging · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A cost-effective immunosensor for the detection and isolation of dental pulp stem cells (DPSCs) based on a quartz crystal microbalance (QCM) has been developed. The recognition mechanism relies on anti-CD34 antibodies, DPSC-specific monoclonal antibodies that are anchored on the surface of the quartz crystals. Due to its high specificity, real time detection, and low cost, the proposed technology has a promising potential in the field of cell biology, for the simultaneous detection and sorting of stem cells from heterogeneous cell samples. The QCM surface was properly tailored through a biotinylated self-assembled monolayer (SAM). The biotin-avidin interaction was used to immobilize the biotinylated anti-CD34 antibody on the gold-coated quartz crystal. After antibody immobilization, a cellular pellet, with a mixed cell population, was analyzed; the results indicated that the developed QCM immunosensor is highly specific, being able to detect and sort only CD34+ cells. Our study suggests that the proposed technology can detect and efficiently sort any kind of cell from samples with high complexity, being simple, selective, and providing for more convenient and time-saving operations.

Indexed as

Quartz Crystal Microbalance TechniquesBiosensing TechniquesGoldImmunoassayQuartzStem CellsGoldQuartzanti-CD34 antibodydental pulpQCM immunosensorself-assembled monolayerstem cells

Identifiers

PMID29182568
PMCPMC5751627
OpenAlexW2770460892

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.