Evidence map›Paper›PMID 29182425›Full record

ArticlePlatelets2019

Endothelial alterations in a canine model of immune thrombocytopenia.

Dana N LeVine, Rachel E Cianciolo, Keith E Linder, Petra Bizikova, Adam J Birkenheuer, Marjory B Brooks, Abdelghaffar K Salous, Shila K Nordone, Dwight A Bellinger, Henry Marr and 5 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Platelets, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.9field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 23 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Review
  5. CanineBiology · 2021
    Article
  6. Article
  7. Endothelial Activation and Immune Thrombocytopenia: An Engagement Waiting for Consolidation.Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 6 institutions in 1 country.

Dana N LeVinea Department of Veterinary Clinical Sciences , Iowa State University , Ames , IA , USA.
Rachel E Ciancioloc Department of Veterinary Biosciences , The Ohio State University , Columbus , OH , USA.
Keith E Linderd Department of Population Health and Pathobiology , North Carolina State University, College of Veterinary Medicine , Raleigh , NC , USA.
Petra Bizikovab Department of Clinical Sciences , North Carolina State University, College of Veterinary Medicine , Raleigh , NC , USA.
Adam J Birkenheuerb Department of Clinical Sciences , North Carolina State University, College of Veterinary Medicine , Raleigh , NC , USA.
Marjory B Brookse Department of Population Medicine and Diagnostic Sciences , Cornell University, College of Veterinary Medicine , Ithaca , NY , USA.
Abdelghaffar K Salousf Division of Cardiovascular Medicine , The Gill Heart Institute, University of Kentucky , Lexington , KY , USA.
Shila K Nordoneg Department of Molecular Biomedical Sciences , North Carolina State University, College of Veterinary Medicine , Raleigh , NC , USA.
Dwight A Bellingerh Department of Pathology and Laboratory Animal Medicine , University of North Carolina , Chapel Hill , NC , USA.
Henry Marrb Department of Clinical Sciences , North Carolina State University, College of Veterinary Medicine , Raleigh , NC , USA.
Sam L Jonesb Department of Clinical Sciences , North Carolina State University, College of Veterinary Medicine , Raleigh , NC , USA.
Thomas H Fischerh Department of Pathology and Laboratory Animal Medicine , University of North Carolina , Chapel Hill , NC , USA.
Yu Dengi Department of Biostatistics , University of North Carolina , Chapel Hill , NC , USA.
Marshall Mazepah Department of Pathology and Laboratory Animal Medicine , University of North Carolina , Chapel Hill , NC , USA.
Nigel S Keyh Department of Pathology and Laboratory Animal Medicine , University of North Carolina , Chapel Hill , NC , USA.
North Carolina State University · USUniversity of North Carolina at Chapel Hill · USCornell University · USIowa State University · USThe Ohio State University · USUniversity of Kentucky · US

Funding

Research Training in Hematology at UNC Chapel HillT32HL007149 · NHLBI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Nigel S. Key · 1985 to 2026
$8.6M
NHLBI NIH HHS T32 HL007149
6 · The paper itself

Abstract

Bleeding heterogeneity amongst patients with immune thrombocytopenia (ITP) is poorly understood. Platelets play a role in maintaining endothelial integrity, and variable thrombocytopenia-induced endothelial changes may influence bleeding severity. Platelet-derived endothelial stabilizers and markers of endothelial integrity in ITP are largely underexplored. We hypothesized that, in a canine ITP model, thrombocytopenia would lead to alterations in the endothelial ultrastructure and that the Von Willebrand factor (vWF) would serve as a marker of endothelial injury associated with thrombocytopenia. Thrombocytopenia was induced in healthy dogs with an antiplatelet antibody infusion; control dogs received an isotype control antibody. Cutaneous biopsies were obtained prior to thrombocytopenia induction, at platelet nadir, 24 hours after nadir, and on platelet recovery. Cutaneous capillaries were assessed by electron microscopy for vessel thickness, the number of pinocytotic vesicles, the number of large vacuoles, and the number of gaps between cells. Pinocytotic vesicles are thought to represent an endothelial membrane reserve that can be used for repair of damaged endothelial cells. Plasma samples were assessed for vWF. ITP dogs had significantly decreased pinocytotic vesicle numbers compared to control dogs (P = 0.0357) and the increase in plasma vWF from baseline to 24 hours correlated directly with the endothelial large vacuole score (R = 0.99103; P < 0.0001). This direct correlation between plasma vWF and the number of large vacuoles, representing the vesiculo-vacuolar organelle (VVO), a permeability structure, suggests that circulating vWF could serve as a biomarker for endothelial alterations and potentially a predictor of thrombocytopenic bleeding. Overall, our results indicate that endothelial damage occurs in the canine ITP model and variability in the degree of endothelial damage may account for differences in the bleeding phenotype among patients with ITP.

Indexed as

AnimalsBiomarkersBiopsyBlood CoagulationBlood PlateletsDisease Models, AnimalDogsEndotheliumFlow CytometryLysophospholipidsMalePlatelet ActivationPlatelet CountPurpura, Thrombocytopenic, IdiopathicSphingosinevon Willebrand FactorBiomarkersLysophospholipidsSphingosinesphingosine 1-phosphatevon Willebrand FactorDogendotheliumITP

Identifiers

PMID29182425
PMCPMC6355380
OpenAlexW2770036465

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.