ArticleBeilstein journal of nanotechnology2017
Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging.
Article in Beilstein journal of nanotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Fluorescence dye-conjugated magnetic core-shell silica nanoparticles for enhanced nucleic acid visualization.RSC advances · 2025Article
- Measuring the Adsorption Cross Section of YOYO-1 to Immobilized DNA Molecules.The journal of physical chemistry. B · 2024Article
- Effect of Flow Rate and Ionic Strength on the Stabilities of YOYO-1 and YO-PRO-1 Intercalated in DNA Molecules.The journal of physical chemistry. B · 2023Article
- Simulating stochastic adsorption of diluted solute molecules at interfaces.AIP advances · 2022Article
- Hollow multishell structures exercise temporal-spatial ordering and dynamic smart behaviour.Nature reviews. Chemistry · 2020Review
- In Situ Sensing of Reactive Oxygen Species on Dye-Stained Single DNA Molecules under Illumination.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification.Nature communications · 2019Article
- Ultrafast Transient Absorption Spectra of Photoexcited YOYO-1 molecules call for additional investigations of their fluorescence quenching mechanism.Journal of photochemistry and photobiology. A, Chemistry · 2018Article
- Precise and economic FIB/SEM for CLEM: with 2 nm voxels through mitosis.Histochemistry and cell biology · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Super-resolution imaging of single DNA molecules via point accumulation for imaging in nanoscale topography (PAINT) has great potential to visualize fine DNA structures with nanometer resolution. In a typical PAINT video acquisition, dye molecules (YOYO-1) in solution sparsely bind to the target surfaces (DNA) whose locations can be mathematically determined by fitting their fluorescent point spread function. Many YOYO-1 molecules intercalate into DNA and remain there during imaging, and most of them have to be temporarily or permanently fluorescently bleached, often stochastically, to allow for the visualization of a few fluorescent events per DNA per frame of the video. Thus, controlling the fluorescence on-off rate is important in PAINT. In this paper, we study the photobleaching of YOYO-1 and its correlation with the quality of the PAINT images. At a low excitation laser power density, the photobleaching of YOYO-1 is too slow and a minimum required power density was identified, which can be theoretically predicted with the proposed method in this report.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.