ArticleNature communications2017
Structural basis for TNA synthesis by an engineered TNA polymerase.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 55 citations in OpenAlex.
- Directed evolution of a TNA polymerase identifies independent paths to fidelity and catalysis.Nature communications · 2025Article
- Structural insights into a DNA polymerase reading the xeno nucleic acid HNA.Nucleic acids research · 2025Article
- DNA Replication across α-l-(3'-2')-Threofuranosyl Nucleotides Mediated by Human DNA Polymerase η.Biochemistry · 2024Article
- Acyclic (RNA (New York, N.Y.) · 2023Article
- Thermophilic Nucleic Acid Polymerases and Their Application in Xenobiology.International journal of molecular sciences · 2022Review
- Crystallographic analysis of engineered polymerases synthesizing phosphonomethylthreosyl nucleic acid.Nucleic acids research · 2022Article
- Mutant polymerases capable of 2' fluoro-modified nucleic acid synthesis and amplification with improved accuracy.RSC chemical biology · 2022Article
- Functional Xeno Nucleic Acids for Biomedical Application.Chemical research in Chinese universities · 2022Review
- Nucleic Acids and Their Analogues for Biomedical Applications.Biosensors · 2022Review
- Utility of Multivalent Aptamers to Develop Nanoscale DNA Devices against Surface Receptors.ACS omega · 2021Review
- Selective Prebiotic Synthesis of α-Threofuranosyl Cytidine by Photochemical Anomerization.Angewandte Chemie (International ed. in English) · 2021Article
- Developmental delay with hypotrophy associated with homozygous functionally relevant REV3L variant.Journal of molecular medicine (Berlin, Germany) · 2021Article
- Structural Studies of HNA Substrate Specificity in Mutants of an Archaeal DNA Polymerase Obtained by Directed Evolution.Biomolecules · 2020Article
- Review
- An overview of 25 years of research on Thermococcus kodakarensis, a genetically versatile model organism for archaeal research.Folia microbiologica · 2020Review
- Beyond DNA and RNA: The Expanding Toolbox of Synthetic Genetics.Cold Spring Harbor perspectives in biology · 2019Review
- Nucleoside analogs in the study of the epitranscriptome.Methods (San Diego, Calif.) · 2019Review
- Activation of Innate Immune Responses by a CpG Oligonucleotide Sequence Composed Entirely of Threose Nucleic Acid.Nucleic acid therapeutics · 2019Article
- Article
- Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates.Frontiers in molecular biosciences · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Darwinian evolution experiments carried out on xeno-nucleic acid (XNA) polymers require engineered polymerases that can faithfully and efficiently copy genetic information back and forth between DNA and XNA. However, current XNA polymerases function with inferior activity relative to their natural counterparts. Here, we report five X-ray crystal structures that illustrate the pathway by which α-(L)-threofuranosyl nucleic acid (TNA) triphosphates are selected and extended in a template-dependent manner using a laboratory-evolved polymerase known as Kod-RI. Structural comparison of the apo, binary, open and closed ternary, and translocated product detail an ensemble of interactions and conformational changes required to promote TNA synthesis. Close inspection of the active site in the closed ternary structure reveals a sub-optimal binding geometry that explains the slow rate of catalysis. This key piece of information, which is missing for all naturally occurring archaeal DNA polymerases, provides a framework for engineering new TNA polymerase variants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.