Evidence map›Paper›PMID 29180623›Full record

ArticleScientific reports2017

Genotyping-by-Sequencing Derived High-Density Linkage Map and its Application to QTL Mapping of Flag Leaf Traits in Bread Wheat.

Waseem Hussain, P Stephen Baenziger, Vikas Belamkar, Mary J Guttieri, Jorge P Venegas, Amanda Easterly, Ahmed Sallam, Jesse Poland

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
76citing papers in PubMed, 1 pooled it
26.4field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

76 citing papers in PubMed, 1 synthesis or guideline pooled it, 144 citations in OpenAlex.

  1. Pooled it
  2. Mining ofHorticulture research · 2026
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  5. Uncovering natural variation inFrontiers in plant science · 2026
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  19. Genetic mapping and analysis of candidate leaf color genes in common winter wheat (Molecular breeding : new strategies in plant improvement · 2023
    Article
  20. Review

16 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Waseem HussainDepartment of Agronomy and Horticulture, University of Nebraska, Lincoln, NE, 68583, USA.
P Stephen BaenzigerDepartment of Agronomy and Horticulture, University of Nebraska, Lincoln, NE, 68583, USA. pbaenziger1@unl.edu.
Vikas BelamkarDepartment of Agronomy and Horticulture, University of Nebraska, Lincoln, NE, 68583, USA.
Mary J GuttieriUSDA, Agricultural Research Service, Center for Grain and Animal Health Research, Hard Winter Wheat Genetics Research Unit, 1515 College Avenue, Manhattan, KS, 66502, USA.
Jorge P VenegasDepartment of Agronomy and Horticulture, University of Nebraska, Lincoln, NE, 68583, USA.
Amanda EasterlyDepartment of Agronomy and Horticulture, University of Nebraska, Lincoln, NE, 68583, USA.
Ahmed SallamDepartment of Genetics, Faculty of Agriculture, Assiut University, 71526, Assiut, Egypt.ORCID 0000-0002-7811-728X
Jesse PolandWheat Genetics Resource Center, Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506, USA.ORCID 0000-0002-7856-1399
University of Nebraska–Lincoln · USAgricultural Research Service · USAssiut University · EGKansas State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Winter wheat parents 'Harry' (drought tolerant) and 'Wesley' (drought susceptible) were used to develop a recombinant inbred population with future goals of identifying genomic regions associated with drought tolerance. To precisely map genomic regions, high-density linkage maps are a prerequisite. In this study genotyping-by- sequencing (GBS) was used to construct the high-density linkage map. The map contained 3,641 markers distributed on 21 chromosomes and spanned 1,959 cM with an average distance of 1.8 cM between markers. The constructed linkage map revealed strong collinearity in marker order across 21 chromosomes with POPSEQ-v2.0, which was based on a high-density linkage map. The reliability of the linkage map for QTL mapping was demonstrated by co-localizing the genes to previously mapped genomic regions for two highly heritable traits, chaff color, and leaf cuticular wax. Applicability of linkage map for QTL mapping of three quantitative traits, flag leaf length, width, and area, identified 21 QTLs in four environments, and QTL expression varied across the environments. Two major stable QTLs, one each for flag leaf length (Qfll.hww-7A) and flag leaf width (Qflw.hww-5A) were identified. The map constructed will facilitate QTL and fine mapping of quantitative traits, map-based cloning, comparative mapping, and in marker-assisted wheat breeding endeavors.

Indexed as

Chromosome MappingGenetic LinkageQuantitative Trait LociBreadChromosomes, PlantCrosses, GeneticGene-Environment InteractionGenotypeGenotyping TechniquesHigh-Throughput Nucleotide SequencingInbreedingPhenotypePolymorphism, Single NucleotideReproducibility of ResultsTriticum

Identifiers

PMID29180623
PMCPMC5703991
OpenAlexW2768723904

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.