Evidence map›Paper›PMID 29180379›Full record

ArticleBioscience reports2017

Selection of internal references for qRT-PCR assays of human hepatocellular carcinoma cell lines.

Yang Liu, Zhaoyu Qin, Lili Cai, Lili Zou, Jing Zhao, Fan Zhong

Open access · goldAbstract read
In one paragraph

Article in Bioscience reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
0.4field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 20 citations in OpenAlex.

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  9. Oncology letters · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Yang LiuDepartment of Oncology, Fudan University Pudong Medical Center, 2800 Gongwei Road, Pudong, Shanghai 201399, China.
Zhaoyu QinDepartment of Systems Biology for Medicine, Shanghai Medical College and Institutes of Biomedical Sciences, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China.
Lili CaiDepartment of Systems Biology for Medicine, Shanghai Medical College and Institutes of Biomedical Sciences, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China.
Lili ZouTranslational Neuroscience and Neural Regeneration and Repair Institute/Institute of Cell therapy, The First Hospital of Yichang, Three Gorges University, 8 Daxue Road, Hubei, Yichang 443002, China.
Jing ZhaoDepartment of Systems Biology for Medicine, Shanghai Medical College and Institutes of Biomedical Sciences, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China.
Fan ZhongDepartment of Gastroenterology, Songjiang Hospital Affiliated First People's Hospital, Shanghai Jiao Tong University, Shanghai 201600, China zonefan@163.com.
Shanghai Medical College of Fudan University · CNPudong Medical Center · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Selecting internal references is important for normalizing the loading quantity of samples in quantitative reverse-transcription PCR (qRT-PCR). In the present study, a systematic evaluation of reference genes among nine hepatocellular carcinoma (HCC) cell lines was conducted. After screening the microarray assay data of ten HCC cell lines, 19 candidate reference genes were preselected and then evaluated by qRT-PCR, together with

Indexed as

Gene Expression ProfilingCarcinoma, HepatocellularCell Line, TumorGene Expression Regulation, NeoplasticHumansLiver NeoplasmsNeoplasm ProteinsReal-Time Polymerase Chain ReactionReference StandardsNeoplasm ProteinsACTBHCCinternal referencesqRT-PCRRefFinderSFRS4TFG

Identifiers

PMID29180379
PMCPMC5741833
OpenAlexW2770367173

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.