ArticleOncotarget2017
Genome-wide 5-hydroxymethylcytosine patterns in human spermatogenesis are associated with semen quality.
Article in Oncotarget, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
22 citing papers in PubMed.
- Redox-Genomic Crosstalk: Linking Oxidative Stress, Sperm DNA Fragmentation, and Epigenetics in Personalized Management of Male Infertility.Journal of personalized medicine · 2026Review
- Impact of sperm fractionation on chromosome positioning, chromatin integrity, DNA methylation, and hydroxymethylation level.Cellular & molecular biology letters · 2025Article
- Redox-Driven Epigenetic Modifications in Sperm: Unraveling Paternal Influences on Embryo Development and Transgenerational Health.Antioxidants (Basel, Switzerland) · 2025Review
- Epigenetics of nonobstructive azoospermia.Asian journal of andrology · 2025Review
- Aberrant Expression of TET2 Accounts for DNA Hypomethylation in Varicocele.Cell journal · 2023Article
- Telomere Length in Human Spermatogenic Cells as a New Potential Predictor of Clinical Outcomes in ART Treatment with Intracytoplasmic Injection of Testicular Spermatozoa.International journal of molecular sciences · 2023Article
- A View on Uterine Leiomyoma Genesis through the Prism of Genetic, Epigenetic and Cellular Heterogeneity.International journal of molecular sciences · 2023Review
- Global 5mC and 5hmC DNA Levels in Human Sperm Subpopulations with Differentially Protaminated Chromatin in Normo- and Oligoasthenozoospermic Males.International journal of molecular sciences · 2022Article
- Telomere Length in Chromosomally Normal and Abnormal Miscarriages and Ongoing Pregnancies and Its Association with 5-hydroxymethylcytosine Patterns.International journal of molecular sciences · 2021Article
- Telomere Length in Metaphase Chromosomes of Human Triploid Zygotes.International journal of molecular sciences · 2021Article
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- High-fat diet-induced and genetically inherited obesity differentially alters DNA methylation profile in the germline of adult male rats.Clinical epigenetics · 2020Article
- Environmental Impact on Male (In)Fertility via Epigenetic Route.Journal of clinical medicine · 2020Review
- Environmental Epigenetics and Genome Flexibility: Focus on 5-Hydroxymethylcytosine.International journal of molecular sciences · 2020Review
- Customized MethylC-Capture Sequencing to Evaluate Variation in the Human Sperm DNA Methylome Representative of Altered Folate Metabolism.Environmental health perspectives · 2019Article
- Bisphenol A Exposure and Sperm ACHE Hydroxymethylation in Men.International journal of environmental research and public health · 2019Article
- Reproductive History of a Woman With 8p and 18p Genetic Imbalance and Minor Phenotypic Abnormalities.Frontiers in genetics · 2019Article
- On the Complexity of Mechanisms and Consequences of Chromothripsis: An Update.Frontiers in genetics · 2019Review
- Epigenetic DNA Modifications Are Correlated With B Chromosomes and Sex in the CichlidFrontiers in genetics · 2019Article
Corrections and comments
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Authors and funding
18 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We performed immunofluorescent analysis of DNA hydroxymethylation and methylation in human testicular spermatogenic cells from azoospermic patients and ejaculated spermatozoa from sperm donors and patients from infertile couples. In contrast to methylation which was present throughout spermatogenesis, hydroxymethylation was either high or almost undetectable in both spermatogenic cells and ejaculated spermatozoa. On testicular cytogenetic preparations, 5-hydroxymethylcytosine was undetectable in mitotic and meiotic chromosomes, and was present exclusively in interphase spermatogonia Ad and in a minor spermatid population. The proportions of hydroxymethylated and non-hydroxymethylated diploid and haploid nuclei were similar among samples, suggesting that the observed alterations of 5-hydroxymethylcytosine patterns in differentiating spermatogenic cells are programmed. In ejaculates, a few spermatozoa had high 5-hydroxymethylcytosine level, while in the other ones hydroxymethylation was almost undetectable. The percentage of highly hydroxymethylated (5-hydroxymethylcytosine-positive) spermatozoa varied strongly among individuals. In patients from infertile couples, it was higher than in sperm donors (
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