ArticleMolecules and cells2017
Targeted Base Editing via RNA-Guided Cytidine Deaminases in Xenopus laevis Embryos.
Article in Molecules and cells, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 38 citations in OpenAlex.
- Tyrosinase-Deficient Skin Melanophore Lineage inInternational journal of molecular sciences · 2026Article
- CRISPR-Mediated Gene Editing: Generating Gene Knockouts or Precision Base Changes in FMethods in molecular biology (Clifton, N.J.) · 2026Article
- Research progress of base editing and prime editing tools based on the CRISPR/Cas system.Molecular therapy. Nucleic acids · 2025Review
- CRISPR-based functional genomics tools in vertebrate models.Experimental & molecular medicine · 2025Review
- Genome editing: An insight into disease resistance, production efficiency, and biomedical applications in livestock.Functional & integrative genomics · 2024Review
- Modelling human genetic disorders in Xenopus tropicalis.Disease models & mechanisms · 2024Review
- Highly efficient genome editing via CRISPR-Cas9 ribonucleoprotein (RNP) delivery in mesenchymal stem cells.BMB reports · 2024Article
- Precise mutagenesis in zebrafish using cytosine base editors.Nature protocols · 2023Review
- Base editors: development and applications in biomedicine.Frontiers of medicine · 2023Review
- One-step base editing in multiple genes by direct embryo injection for pig trait improvement.Science China. Life sciences · 2022Article
- Base editing and prime editing in laboratory animals.Laboratory animals · 2022Article
- Precision genome editing using cytosine and adenine base editors in mammalian cells.Nature protocols · 2021Article
- Base and Prime Editing Technologies for Blood Disorders.Frontiers in genome editing · 2021Review
- Base editing: advances and therapeutic opportunities.Nature reviews. Drug discovery · 2020Review
- Genome editing with CRISPR-Cas nucleases, base editors, transposases and prime editors.Nature biotechnology · 2020Review
- Article
- Advances in engineering CRISPR-Cas9 as a molecular Swiss Army knife.Synthetic biology (Oxford, England) · 2020Review
- Gene Editing and Alzheimer's Disease: Is There Light at the End of the Tunnel?Frontiers in genome editing · 2020Review
- Cas9 Ribonucleoprotein Complex Delivery: Methods and Applications for Neuroinflammation.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2019Review
- Dead Cas Systems: Types, Principles, and Applications.International journal of molecular sciences · 2019Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome editing using programmable nucleases such as CRISPR/Cas9 or Cpf1 has emerged as powerful tools for gene knock-out or knock-in in various organisms. While most genetic diseases are caused by point mutations, these genome-editing approaches are inefficient in inducing single-nucleotide substitutions. Recently, Cas9-linked cytidine deaminases, named base editors (BEs), have been shown to convert cytidine to uridine efficiently, leading to targeted single-base pair substitutions in human cells and organisms. Here, we first report on the generation of Xenopus laevis mutants with targeted single-base pair substitutions using this RNA-guided programmable deaminase. Injection of base editor 3 (BE3) ribonucleoprotein targeting the tyrosinase (tyr) gene in early embryos can induce site-specific base conversions with the rates of up to 20.5%, resulting in oculocutaneous albinism phenotypes without off-target mutations. We further test this base-editing system by targeting the tp53 gene with the result that the expected single-base pair substitutions are observed at the target site. Collectively, these data establish that the programmable deaminases are efficient tools for creating targeted point mutations for human disease modeling in Xenopus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.