ArticleBMC veterinary research2017
Reference gene validation for gene expression normalization in canine osteosarcoma: a geNorm algorithm approach.
Article in BMC veterinary research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 25 citations in OpenAlex.
- Evaluation and Selection of Stable Reference Genes for qRT-PCR Analysis in Different Tissues ofAnimals : an open access journal from MDPI · 2026Article
- Evaluation of normalisation strategies for qPCR data obtained from canine gastrointestinal tissues with different pathologies.Scientific reports · 2025Article
- Cilia-deficient renal tubule cells are primed for injury with mitochondrial defects and aberrant tryptophan metabolism.American journal of physiology. Renal physiology · 2024Article
- Programmed Death-Ligand (PD-L1), Epidermal Growth Factor (EGF), Relaxin, and Matrix Metalloproteinase-3 (MMP3): Potential Biomarkers of Malignancy in Canine Mammary Neoplasia.International journal of molecular sciences · 2024Article
- Evaluation of Reference Genes for Normalizing RT-qPCR and Analysis of the Expression Patterns ofInternational journal of molecular sciences · 2023Article
- Animal models of Soft Tissue Sarcoma for alternative anticancer therapy studies: characterization of the A-72 Canine Cell Line.Veterinary research communications · 2023Article
- Antioxidant 1 copper chaperone gene expression and copper levels in dog osteosarcoma patients.Veterinary and comparative oncology · 2023Article
- Validation of droplet digital PCR for cytokeratin 19 mRNA detection in canine peripheral blood and mammary gland.Scientific reports · 2022Article
- The Effect of Arginase on Canine T-Lymphocyte Functions and its Modulation by All-Trans Retinoid Acid (ATRA) in Canine Monocyte-Derived Macrophages.Veterinary sciences · 2022Article
- The Australian dingo is an early offshoot of modern breed dogs.Science advances · 2022Article
- Novel application of single-cell next-generation sequencing for determination of intratumoral heterogeneity of canine osteosarcoma cell lines.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2021Article
- Characterization of D-17 Canine Osteosarcoma Cell Line and Evaluation of Its Ability to Response to Infective Stressor Used as Alternative Anticancer Therapy.Animals : an open access journal from MDPI · 2020Article
- Optimal reference genes for gene expression analysis in polyploid of Cyprinus carpio and Carassius auratus.BMC genetics · 2020Article
- S100A4 mRNA-protein relationship uncovered by measurement noise reduction.Journal of molecular medicine (Berlin, Germany) · 2020Article
- The interaction between RUNX2 and core binding factor beta as a potential therapeutic target in canine osteosarcoma.Veterinary and comparative oncology · 2020Article
- Characterization of MDCK cells and evaluation of their ability to respond to infectious and non-infectious stressors.Cytotechnology · 2020Article
- Impact of 5 fluorouracil chemotherapy on gut inflammation, functional parameters, and gut microbiota.Brain, behavior, and immunity · 2019Article
- Selection of Reliable Reference Genes for RT-qPCR Analysis ofFrontiers in genetics · 2018Article
- A genetically engineered microRNA-34a prodrug demonstrates anti-tumor activity in a canine model of osteosarcoma.PloS one · 2018Article
- MicroRNA Expression in the Infarcted Heart Following Neonatal Cardiovascular Progenitor Cell Transplantation in a Sheep Model of Stem Cell-Based Repair.Cell transplantationArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundQuantitative PCR (qPCR) is a common method for quantifying mRNA expression. Given the heterogeneity present in tumor tissues, it is crucial to normalize target mRNA expression data using appropriate reference genes that are stably expressed under a variety of pathological and experimental conditions. No studies have validated specific reference genes in canine osteosarcoma (OS). Previous gene expression studies involving canine OS have used one or two reference genes to normalize gene expression. This study aimed to validate a panel of reference genes commonly used for normalization of canine OS gene expression data using the geNorm algorithm. qPCR analysis of nine canine reference genes was performed on 40 snap-frozen primary OS tumors and seven cell lines.
resultsTumors with a variety of clinical and pathological characteristics were selected. Gene expression stability and the optimal number of reference genes for gene expression normalization were calculated. RPS5 and HNRNPH were highly stable among OS cell lines, while RPS5 and RPS19 were the best combination for primary tumors. Pairwise variation analysis recommended four and two reference genes for optimal normalization of the expression data of canine OS tumors and cell lines, respectively.
conclusionsAppropriate combinations of reference genes are recommended to normalize mRNA levels in canine OS tumors and cell lines to facilitate standardized and reliable quantification of target gene expression, which is essential for investigating key genes involved in canine OS metastasis and for comparative biomarker discovery.
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