ArticleCell and tissue research2018
Live imaging analysis of human gastric epithelial spheroids reveals spontaneous rupture, rotation and fusion events.
Article in Cell and tissue research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 46 citations in OpenAlex.
- Probing the Luminal Compartment of 3D Organoids via Particle Tracking Microrheology.Bio-protocol · 2026Article
- Physical, chemical, and structural properties of human gastric organoid-derived mucus.American journal of physiology. Gastrointestinal and liver physiology · 2026Article
- Leveraging organoid models to understand mechanisms of viral infections and immunity in bats.Disease models & mechanisms · 2026Review
- Physical, chemical, and structural properties of human gastric organoid-derived mucus.bioRxiv : the preprint server for biology · 2025Article
- Spermine oxidase promotes Helicobacter pylori-mediated gastric carcinogenesis through acrolein production.Oncogene · 2025Article
- Modeling the pre-metastatic niche of gastric cancer peritoneal metastasis under spatiotemporal resolution and investigating EVs-mediated immune suppression.Frontiers in immunology · 2025Review
- Applying 3D cultures and high-throughput technologies to study host-pathogen interactions.Frontiers in immunology · 2025Review
- Antiviral responses in a Jamaican fruit bat intestinal organoid model of SARS-CoV-2 infection.Nature communications · 2023Article
- Organoids as host models for infection biology - a review of methods.Experimental & molecular medicine · 2021Review
- A High-Throughput Metabolic Microarray Assay Reveals Antibacterial Effects of Black and Red Raspberries and Blackberries againstAntibiotics (Basel, Switzerland) · 2021Article
- Bio-assembling Macro-Scale, Lumenized Airway Tubes of Defined Shape via Multi-Organoid Patterning and Fusion.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis.BMC biology · 2021Article
- A Synthetic Hydrogel, VitroGelFrontiers in pharmacology · 2021Article
- Article
- Functional Properties of Helicobacter pylori VacA Toxin m1 and m2 Variants.Infection and immunity · 2020Article
- Long-term flow through human intestinal organoids with the gut organoid flow chip (GOFlowChip).Lab on a chip · 2019Article
- Slug and Snail have differential effects in directing colonic epithelial wound healing and partially mediate the restitutive effects of butyrate.American journal of physiology. Gastrointestinal and liver physiology · 2019Article
- The migration and fusion events related to ROCK activity strongly influence the morphology of chicken embryo intestinal organoids.Protoplasma · 2019Article
- A Novel Gastric Spheroid Co-culture Model Reveals Chemokine-Dependent Recruitment of Human Dendritic Cells to the Gastric Epithelium.Cellular and molecular gastroenterology and hepatology · 2019Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
Three-dimensional cultures of primary epithelial cells including organoids, enteroids and epithelial spheroids have become increasingly popular for studies of gastrointestinal development, mucosal immunology and epithelial infection. However, little is known about the behavior of these complex cultures in their three-dimensional culture matrix. Therefore, we performed extended time-lapse imaging analysis (up to 4 days) of human gastric epithelial spheroids generated from adult tissue samples in order to visualize the dynamics of the spheroids in detail. Human gastric epithelial spheroids cultured in our laboratory grew to an average diameter of 443.9 ± 34.6 μm after 12 days, with the largest spheroids reaching diameters of >1000 μm. Live imaging analysis revealed that spheroid growth was associated with cyclic rupture of the epithelial shell at a frequency of 0.32 ± 0.1/day, which led to the release of luminal contents. Spheroid rupture usually resulted in an initial collapse, followed by spontaneous re-formation of the spheres. Moreover, spheroids frequently rotated around their axes within the Matrigel matrix, possibly propelled by basolateral pseudopodia-like formations of the epithelial cells. Interestingly, adjacent spheroids occasionally underwent luminal fusion, as visualized by injection of individual spheroids with FITC-Dextran (4 kDa). In summary, our analysis revealed unexpected dynamics in human gastric spheroids that challenge our current view of cultured epithelia as static entities and that may need to be considered when performing spheroid infection experiments.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.