ArticlePloS one2017
Plant-type phytoene desaturase: Functional evaluation of structural implications.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- Article
- Targeting a conserved functional motif in the PDS gene enables efficient CRISPR/Cas9 editing in banana.Scientific reports · 2026Article
- Establishment of a TRV-based virus-induced gene silencing system using the vacuum infiltration in Tartary buckwheat.Plant cell reports · 2025Article
- Proteomic analysis of differential responses to norflurazon herbicide in the model green alga Chlamydomonas reinhardtii.Scientific reports · 2025Article
- Genome-wide identification of VOZ gene family in ten cotton species and the function analysis of GhVOZ2 involved in heat stress response.BMC genomics · 2025Article
- Maize transcriptome profiling reveals low temperatures affect photosynthesis during the emergence stage.Frontiers in plant science · 2025Article
- Rapid and efficient in planta genome editing in sorghum using foxtail mosaic virus-mediated sgRNA delivery.The Plant journal : for cell and molecular biology · 2025Article
- Combining systems and synthetic biology for in vivo enzymology.The EMBO journal · 2024Article
- CRISPR/Cas9 based genome editing of Phytoene desaturase (PDS) gene in chilli pepper (Capsicum annuum L.).Journal, genetic engineering & biotechnology · 2024Article
- Jasmonate ZIM Domain Protein (Plants (Basel, Switzerland) · 2024Article
- Multiplex CRISPR/Cas9-mediated knockout of the phytoene desaturase gene in Coffea canephora.Scientific reports · 2022Article
- Advancement of Phenoxypyridine as an Active Scaffold for Pesticides.Molecules (Basel, Switzerland) · 2022Review
- Exogenous Gibberellin Treatment Enhances Melatonin Synthesis for Melatonin-Enriched Rice Production.Biomolecules · 2022Article
- Knockout of phytoene desaturase gene using CRISPR/Cas9 in highbush blueberry.Frontiers in plant science · 2022Article
- Proteomics and Interspecies Interaction Analysis Revealed Abscisic Acid Signalling to Be the Primary Driver for Oil Palm's Response against Red Palm Weevil Infestation.Plants (Basel, Switzerland) · 2021Article
- Mitosis Inhibitors Induce Massive Accumulation of Phytoene in the MicroalgaMarine drugs · 2021Article
- Improved Sample Preparation and Optimized Solvent Extraction for Quantitation of Carotenoids.Plant foods for human nutrition (Dordrecht, Netherlands) · 2021Article
- Soil Acidobacteria Strain AB23 Resistance to Oxidative Stress Through Production of Carotenoids.Microbial ecology · 2021Article
- Multiplicity of carotene patterns derives from competition between phytoene desaturase diversification and biological environments.Scientific reports · 2020Article
- Bioinformatic Analysis of the Flavin-Dependent Amine Oxidase Superfamily: Adaptations for Substrate Specificity and Catalytic Diversity.Journal of molecular biology · 2020Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phytoene desaturase (PDS) is an essential plant carotenoid biosynthetic enzyme and a prominent target of certain inhibitors, such as norflurazon, acting as bleaching herbicides. PDS catalyzes the introduction of two double bonds into 15-cis-phytoene, yielding 9,15,9'-tri-cis-ζ-carotene via the intermediate 9,15-di-cis-phytofluene. We present the necessary data to scrutinize functional implications inferred from the recently resolved crystal structure of Oryza sativa PDS in a complex with norflurazon. Using dynamic mathematical modeling of reaction time courses, we support the relevance of homotetrameric assembly of the enzyme observed in crystallo by providing evidence for substrate channeling of the intermediate phytofluene between individual subunits at membrane surfaces. Kinetic investigations are compatible with an ordered ping-pong bi-bi kinetic mechanism in which the carotene and the quinone electron acceptor successively occupy the same catalytic site. The mutagenesis of a conserved arginine that forms a hydrogen bond with norflurazon, the latter competing with plastoquinone, corroborates the possibility of engineering herbicide resistance, however, at the expense of diminished catalytic activity. This mutagenesis also supports a "flavin only" mechanism of carotene desaturation not requiring charged residues in the active site. Evidence for the role of the central 15-cis double bond of phytoene in determining regio-specificity of carotene desaturation is presented.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.