Evidence map›Paper›PMID 29176801›Full record

ArticlePloS one2017

Increasing vaccine production using pulsed ultrasound waves.

Jida Xing, Shrishti Singh, Yupeng Zhao, Yan Duan, Huining Guo, Chenxia Hu, Allan Ma, Rajan George, James Z Xing, Ankarao Kalluri and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 3 countries.

Jida XingDepartment of Electrical and Computer Engineering, University of Alberta, Edmonton, Canada.
Shrishti SinghDepartment of Biomedical Engineering, University of Bridgeport, Bridgeport, Connecticut, United States of America.
Yupeng ZhaoDepartment of Biomedical Engineering, University of Alberta, Edmonton, Canada.
Yan DuanDepartment of Electrical and Computer Engineering, University of British Columbia, Vancouver, Canada.
Huining GuoDepartment of Physiatry, University of Alberta, Edmonton, Canada.
Chenxia HuSchool of Chinese Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou, China.
Allan MaAkshaya Bio Inc., Edmonton, Alberta, Canada.
Rajan GeorgeAkshaya Bio Inc., Edmonton, Alberta, Canada.
James Z XingDepartment of Laboratory Medicine & Pathology, University of Alberta, Edmonton, Canada.
Ankarao KalluriDepartment of Biomedical Engineering, University of Bridgeport, Bridgeport, Connecticut, United States of America.
Isaac MacwanDepartment of Biomedical Engineering, University of Bridgeport, Bridgeport, Connecticut, United States of America.
Prabir PatraDepartment of Biomedical Engineering, University of Bridgeport, Bridgeport, Connecticut, United States of America.
Jie ChenDepartment of Electrical and Computer Engineering, University of Alberta, Edmonton, Canada.ORCID http://orcid.org/0000-0001-7925-3729
University of Alberta · CAUniversity of Bridgeport · USGuangzhou University of Chinese Medicine · CNUniversity of British Columbia · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Vaccination is a safe and effective approach to prevent deadly diseases. To increase vaccine production, we propose that a mechanical stimulation can enhance protein production. In order to prove this hypothesis, Sf9 insect cells were used to evaluate the increase in the expression of a fusion protein from hepatitis B virus (HBV S1/S2). We discovered that the ultrasound stimulation at a frequency of 1.5 MHz, intensity of 60 mW/cm2, for a duration of 10 minutes per day increased HBV S1/S2 by 27%. We further derived a model for transport through a cell membrane under the effect of ultrasound waves, tested the key assumptions of the model through a molecular dynamics simulation package, NAMD (Nanoscale Molecular Dynamics program) and utilized CHARMM force field in a steered molecular dynamics environment. The results show that ultrasound waves can increase cell permeability, which, in turn, can enhance nutrient / waste exchange thus leading to enhanced vaccine production. This finding is very meaningful in either shortening vaccine production time, or increasing the yield of proteins for use as vaccines.

Indexed as

Ultrasonic Waves4-Chloro-7-nitrobenzofurazanAnimalsBlotting, WesternCell Membrane PermeabilityDeoxyglucoseHepatitis B VaccinesMolecular Dynamics SimulationPhosphatidylcholinesProteinsSf9 CellsSonicationThermodynamics1,2-oleoylphosphatidylcholine2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose4-Chloro-7-nitrobenzofurazanDeoxyglucoseHepatitis B VaccinesPhosphatidylcholinesProteins

Identifiers

PMID29176801
PMCPMC5703500
OpenAlexW2769305996

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.