ArticleScientific reports2017
Exploration of Protein Unfolding by Modelling Calorimetry Data from Reheating.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 58 citations in OpenAlex.
- Cysteine-mediated structural stabilization of the tetrameric GlpF.European biophysics journal : EBJ · 2026Article
- pH-Dependent Stability and Cooperativity of Protein Unfolding from Differential Scanning Calorimetry Using FitFoldData.Biomolecules · 2026Article
- Thermophysical Properties of a tRNA Isodecoder.The journal of physical chemistry. B · 2026Article
- FitFoldData: A Web-Based Toolkit for Circular Dichroism and Differential Scanning Calorimetry Data Analysis of Protein Thermal Denaturation.ACS omega · 2025Article
- ChiraKit: an online tool for the analysis of circular dichroism spectroscopy data.Nucleic acids research · 2025Article
- Developing New Peptides and Peptide-Drug Conjugates for Targeting the FGFR2 Receptor-Expressing Tumor Cells and 3D Spheroids.Biomimetics (Basel, Switzerland) · 2024Article
- Programming aliphatic polyester degradation by engineered bacterial spores.bioRxiv : the preprint server for biology · 2024Article
- Phase-Dependent Adsorption of Myelin Basic Protein to Phosphatidylcholine Lipid Bilayers.Membranes · 2024Article
- A thermodynamic investigation into protein-excipient interactions involving different grades of polysorbate 20 and 80.Journal of thermal analysis and calorimetry · 2024Article
- Biophysical Characterization of Membrane Proteins.Methods in molecular biology (Clifton, N.J.) · 2023Article
- A circular dichroism study of the protective role of polyphosphoesters polymer chains in polyphosphoester-myoglobin conjugates.Chirality · 2022Article
- Expression and characterization of a family 45 glycosyl hydrolase from Fomitopsis pinicola and comparison to Phanerochaete chrysosporium Cel45A.Enzyme and microbial technology · 2022Article
- Seeking Solvation: Exploring the Role of Protein Hydration in Silk Gelation.Molecules (Basel, Switzerland) · 2022Article
- Aerosol capture and coronavirus spike protein deactivation by enzyme functionalized antiviral membranes.Communications materials · 2022Article
- Expanding the toolbox for predictive parameters describing antibody stability considering thermodynamic and kinetic determinants.Pharmaceutical research · 2021Article
- Cation folding and the thermal stability limit of the ionic liquid [BMIMRSC advances · 2021Article
- In-depth interrogation of protein thermal unfolding data with MoltenProt.Protein science : a publication of the Protein Society · 2021Article
- The Effect of Dimethyl Sulfoxide on the Lysozyme Unfolding Kinetics, Thermodynamics, and Mechanism.Biomolecules · 2019Article
- A look back at the molten globule state of proteins: thermodynamic aspects.Biophysical reviews · 2019Review
- CalFitter: a web server for analysis of protein thermal denaturation data.Nucleic acids research · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Studies of protein unfolding mechanisms are critical for understanding protein functions inside cells, de novo protein design as well as defining the role of protein misfolding in neurodegenerative disorders. Calorimetry has proven indispensable in this regard for recording full energetic profiles of protein unfolding and permitting data fitting based on unfolding pathway models. While both kinetic and thermodynamic protein stability are analysed by varying scan rates and reheating, the latter is rarely used in curve-fitting, leading to a significant loss of information from experiments. To extract this information, we propose fitting both first and second scans simultaneously. Four most common single-peak transition models are considered: (i) fully reversible, (ii) fully irreversible, (iii) partially reversible transitions, and (iv) general three-state models. The method is validated using calorimetry data for chicken egg lysozyme, mutated Protein A, three wild-types of haloalkane dehalogenases, and a mutant stabilized by protein engineering. We show that modelling of reheating increases the precision of determination of unfolding mechanisms, free energies, temperatures, and heat capacity differences. Moreover, this modelling indicates whether alternative refolding pathways might occur upon cooling. The Matlab-based data fitting software tool and its user guide are provided as a supplement.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.