ArticleScientific reports2017
Visualising the 3D microstructure of stained and native intervertebral discs using X-ray microtomography.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Stepwise micro-CT analysis of sample processing effects on the 3D morphology of human intervertebral discs.Osteoarthritis and cartilage open · 2026Article
- 3D anatomical atlas of the heads of male and female adult Chamaeleo calyptratus.Anatomical record (Hoboken, N.J. : 2007) · 2026Article
- High-Resolution 3-Dimensional Micro-CT Imaging of Intervertebral Discs Using a Novel Contrast Agent.JOR spine · 2025Article
- An overview of reliable and representative DVC measurements for musculoskeletal tissues.Journal of microscopy · 2025Review
- Microstructural imaging of the cartilaginous endplate in the intervertebral disc.Osteoarthritis imaging · 2025Article
- Spatial and temporal evaluation of iodine uptake and radiodensity in meniscus tissue using contrast-enhanced micro-CT.Heliyon · 2024Article
- Seeing the unseen: The role of bioimaging techniques for the diagnostic interventions in intervertebral disc degeneration.Bone reports · 2024Review
- Article
- Does Vitamin K2 Influence the Interplay between Diabetes Mellitus and Intervertebral Disc Degeneration in a Rat Model?Nutrients · 2023Article
- Heterotopic mineral deposits in intact rat Achilles tendons are characterized by a unique fiber-like structure.Journal of structural biology: X · 2023Article
- Importance of Matrix Cues on Intervertebral Disc Development, Degeneration, and Regeneration.International journal of molecular sciences · 2022Review
- Regional variations in discrete collagen fibre mechanics within intact intervertebral disc resolved using synchrotron computed tomography and digital volume correlation.Acta biomaterialia · 2022Article
- A quality optimization approach to image Achilles tendon microstructure by phase-contrast enhanced synchrotron micro-tomography.Scientific reports · 2021Article
- Engineering multifunctional metal/protein hybrid nanomaterials as tools for therapeutic intervention and high-sensitivity detection.Chemical science · 2020Article
- Phosphotungstic acid-enhanced microCT: Optimized protocols for embryonic and early postnatal mice.Developmental dynamics : an official publication of the American Association of Anatomists · 2020Article
- X-ray computed tomography in life sciences.BMC biology · 2020Review
- Polylysine Enriched Matrices: A Promising Approach for Vascular Grafts.Frontiers in bioengineering and biotechnology · 2020Article
- 3D Muscle Architecture of the Pectoral Muscles of European Starling (Integrative organismal biology (Oxford, England) · 2019Article
- MicroCT imaging reveals differential 3D micro-scale remodelling of the murine aorta in ageing and Marfan syndrome.Theranostics · 2018Article
- Quantification of porcine lower thoracic spinal cord morphology with intact dura mater using high-resolution μCT.Journal of neuroimaging : official journal of the American Society of NeuroimagingArticle
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intervertebral disc degeneration (IVDD) is linked to low back pain. Microstructural changes during degeneration have previously been imaged using 2D sectioning techniques and 3D methods which are limited to small specimens and prone to inducing artefacts from sample preparation. This study explores micro computed X-ray tomography (microCT) methods with the aim of resolving IVD 3D microstructure whilst minimising sample preparation artefacts. Low X-ray absorption contrast in non-mineralised tissue can be enhanced using staining and phase contrast techniques. A step-wise approach, including comparing three stains, was used to develop microCT for bovine tail IVD using laboratory and synchrotron sources. Staining successfully contrasted collagenous structures; however not all regions were stained and the procedure induced macroscopic structural changes. Phase contrast microCT of chemically fixed yet unstained samples resolved the nucleus pulposus, annulus fibrosus and constituent lamellae, and finer structures including collagen bundles and cross-bridges. Using the same imaging methods native tissue scans were of slightly lower contrast but free from sample processing artefacts. In the future these methods may be used to characterise structural remodelling in soft (non-calcified) tissues and to conduct in situ studies of native loaded tissues and constructs to characterise their 3D mechanical properties.
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Registered trials
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