Evidence map›Paper›PMID 29174541›Full record

ArticleCell chemical biology2018

Engineered Multivalent Sensors to Detect Coexisting Histone Modifications in Living Stem Cells.

Aurore M-F Delachat, Nora Guidotti, Andreas L Bachmann, Antonio C A Meireles-Filho, Horst Pick, Carolin C Lechner, Cédric Deluz, Bart Deplancke, David M Suter, Beat Fierz

Abstract read
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In one paragraph

Article in Cell chemical biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed.

  1. Review
  2. Histone bivalency in CNS development.Genes & development · 2025
    Review
  3. Article
  4. Article
  5. Article
  6. Review
  7. Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Studying Chromatin Epigenetics with Fluorescence Microscopy.International journal of molecular sciences · 2022
    Review
  14. Review
  15. Development and application of novel BiFC probes for cell sorting based on epigenetic modification.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2022
    Article
  16. Review
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Aurore M-F DelachatLaboratory of Biophysical Chemistry of Macromolecules (LCBM), Institute of Chemical Sciences and Engineering (ISIC), Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.
Nora GuidottiLaboratory of Biophysical Chemistry of Macromolecules (LCBM), Institute of Chemical Sciences and Engineering (ISIC), Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.
Andreas L BachmannLaboratory of Biophysical Chemistry of Macromolecules (LCBM), Institute of Chemical Sciences and Engineering (ISIC), Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.
Antonio C A Meireles-FilhoLaboratory of Systems Biology and Genetics, Institute of Bioengineering (IBI), School of Life Sciences, EPFL and Swiss Institute of Bioinformatics (SIB), 1015 Lausanne, Switzerland.
Horst PickLaboratory of Biophysical Chemistry of Macromolecules (LCBM), Institute of Chemical Sciences and Engineering (ISIC), Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.
Carolin C LechnerLaboratory of Biophysical Chemistry of Macromolecules (LCBM), Institute of Chemical Sciences and Engineering (ISIC), Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.
Cédric DeluzUPSUTER, IBI, School of Life Sciences, EPFL, 1015 Lausanne, Switzerland.
Bart DeplanckeLaboratory of Systems Biology and Genetics, Institute of Bioengineering (IBI), School of Life Sciences, EPFL and Swiss Institute of Bioinformatics (SIB), 1015 Lausanne, Switzerland.
David M SuterUPSUTER, IBI, School of Life Sciences, EPFL, 1015 Lausanne, Switzerland.
Beat FierzLaboratory of Biophysical Chemistry of Macromolecules (LCBM), Institute of Chemical Sciences and Engineering (ISIC), Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland. Electronic address: beat.fierz@epfl.ch.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The regulation of fundamental processes such as gene expression or cell differentiation involves chromatin states, demarcated by combinatorial histone post-translational modification (PTM) patterns. The subnuclear organization and dynamics of chromatin states is not well understood, as tools for their detection and modulation in live cells are lacking. Here, we report the development of genetically encoded chromatin-sensing multivalent probes, cMAPs, selective for bivalent chromatin, a PTM pattern associated with pluripotency in embryonic stem cells (ESCs). cMAPs were engineered from a set of PTM-binding (reader) proteins and optimized using synthetic nucleosomes carrying defined PTMs. Applied in live ESCs, cMAPs formed discrete subnuclear foci, revealing the organization of bivalent chromatin into local clusters. Moreover, cMAPs enabled direct monitoring of the loss of bivalency upon treatment with small-molecule epigenetic modulators. cMAPs thus provide a versatile platform to monitor chromatin state dynamics in live cells.

Indexed as

Protein EngineeringChromatinEmbryonic Stem CellsHistonesHumansLuminescent ProteinsMolecular StructureProtein Processing, Post-TranslationalChromatinHistonesLuminescent Proteinsbivalent domainsepigeneticsfluorescent sensorshistone modificationsmultivalent bindingstem cells

Identifiers

PMID29174541

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.