Evidence map›Paper›PMID 29169387›Full record

ArticleParasites & vectors2017

Flotation techniques (FLOTAC and mini-FLOTAC) for detecting gastrointestinal parasites in howler monkeys.

Mayra Alejandra Alvarado-Villalobos, Giuseppe Cringoli, Maria Paola Maurelli, Aurelie Cambou, Laura Rinaldi, Arturo Barbachano-Guerrero, Roger Guevara, Colin A Chapman, Juan Carlos Serio-Silva

Open access · goldAbstract read
In one paragraph

Article in Parasites & vectors, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.1field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Case-report: Massive infection byFrontiers in veterinary science · 2023
    Article
  5. Article
  6. Review
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 4 countries.

Mayra Alejandra Alvarado-VillalobosRed de Biología y Conservación de Vertebrados, Instituto de Ecología A.C, Xalapa, 91070, Veracruz, Mexico.
Giuseppe CringoliUnit of Parasitology and Parasitic Diseases, Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy.
Maria Paola MaurelliUnit of Parasitology and Parasitic Diseases, Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy.
Aurelie CambouENSAIA (Ecole National e Supérieure d'Ágronomie et des Industries Alimentaires), Vandoeuvre-lés-Nancy, France.
Laura RinaldiUnit of Parasitology and Parasitic Diseases, Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy.
Arturo Barbachano-GuerreroLaboratorio de Medicina de Conservación, Departamento de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, México, Mexico.
Roger GuevaraRed de Biología Evolutiva, Instituto de Ecología A.C, Xalapa, 91070, Veracruz, Mexico.
Colin A ChapmanDepartment of Anthropology & McGill School of Environment, McGill University, Quebec, Montreal, H3A 2T7, Canada.
Juan Carlos Serio-SilvaRed de Biología y Conservación de Vertebrados, Instituto de Ecología A.C, Xalapa, 91070, Veracruz, Mexico. juan.serio@inecol.mx.
Instituto de Ecología · MXUniversity of Naples Federico II · ITInstituto Politécnico Nacional · MXLaboratoire Sols et Environnement · FRMcGill University · CA

Funding

CONACYT 280637
6 · The paper itself

Abstract

backgroundAnalyses of environmental correlates of the composition of gastrointestinal parasite communities in black howler monkeys (Alouatta pigra) have been hindered by inadequate calibration techniques of detection and quantification methods of the parasites. Here we calibrate samples and compare the likelihood of parasite detection using two flotation techniques, FLOTAC and Mini-FLOTAC, and compare flotation solution, preservation method and dilution ratio for egg detection and counts of the most common parasites (Controrchis spp. and Trypanoxyuris spp.) in howler monkeys.

resultsFor samples preserved in 5% formalin, the Mini-FLOTAC technique was the best option for qualitative and quantitative copro-microscopic analysis. This technique displays an 83.3% and 100% detection of Controrchis spp. and Trypanoxyuris spp. infections, respectively. For the trematode Controrchis spp., more eggs per gram of feces (EPG) were recorded with the flotation solution (FS) #7 (zinc sulfate; specific gravity SG = 1.35) at 1:20 and 1:25 dilution than other methods. By contrast, for the nematode Trypanoxyuris spp., the best results were recorded with FS1 (sucrose and formaldehyde; SG = 1.20) at 1:10 dilution.

conclusionsWe recommend the Mini-FLOTAC technique for general use with parasite analysis on frugivore/folivores like the howler monkey, especially if many samples are analyzed. The technique has a high detection rate and the best EPG counts, allowing the qualitative and quantitative analysis of parasite load among the species or populations without the need for specialized equipment.

Indexed as

AlouattaAnimalsFecesFormaldehydeGastrointestinal TractIntestinal Diseases, ParasiticNematodaParasite Egg CountParasite LoadSpecimen HandlingSucroseTrematode InfectionsZinc SulfateFormaldehydeSucroseZinc SulfateAlouattaGastrointestinal parasitesHowler monkeysNematodesQuantitative flotationTrematodes

Identifiers

PMID29169387
PMCPMC5701314
OpenAlexW2769925599

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.