ArticleBMC cancer2017
Multi-region and single-cell sequencing reveal variable genomic heterogeneity in rectal cancer.
Article in BMC cancer, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Review
- Single-cell omics: experimental workflow, data analyses and applications.Science China. Life sciences · 2025Review
- CaMutQC: An R package for integrative quality control and filtration of cancer somatic mutations.Computational and structural biotechnology journal · 2025Article
- Application of Single-Cell Sequencing Technology in Research on Colorectal Cancer.Journal of personalized medicine · 2024Review
- Mutant-allele dispersion correlates with prognosis risk in patients with advanced non-small cell lung cancer.Journal of cancer research and clinical oncology · 2023Article
- Genomic landscape of locally advanced rectal adenocarcinoma: Comparison between before and after neoadjuvant chemoradiation and effects of genetic biomarkers on clinical outcomes and tumor response.Cancer medicine · 2023Article
- Application of single-cell sequencing to the research of tumor microenvironment.Frontiers in immunology · 2023Review
- Intestinal cellular heterogeneity and disease development revealed by single-cell technology.Cell regeneration (London, England) · 2022Review
- Single-cell genomic and transcriptomic landscapes of primary and metastatic colorectal cancer tumors.Genome medicine · 2022Article
- Applications and Achievements of Single-Cell Sequencing in Gastrointestinal Cancer.Frontiers in oncology · 2022Review
- Single-Cell RNA Sequencing for Analyzing the Intestinal Tract in Healthy and Diseased Individuals.Frontiers in cell and developmental biology · 2022Review
- A Portrait of Intratumoral Genomic and Transcriptomic Heterogeneity at Single-Cell Level in Colorectal Cancer.Medicina (Kaunas, Lithuania) · 2021Review
- Applications of Single-Cell DNA Sequencing.Annual review of genomics and human genetics · 2021Review
- Article
- Deep Learning for Automatic Subclassification of Gastric Carcinoma Using Whole-Slide Histopathology Images.Cancers · 2021Article
- Article
- MesKit: a tool kit for dissecting cancer evolution of multi-region tumor biopsies through somatic alterations.GigaScience · 2021Article
- Clinical Implications of Colorectal Cancer Stem Cells in the Age of Single-Cell Omics and Targeted Therapies.Gastroenterology · 2021Review
- Intra-Tumor Heterogeneity of Colorectal Cancer Necessitates the Multi-Regional Sequencing for Comprehensive Mutational Profiling.Cancer management and research · 2021Article
- Nutrient metabolism and cancer in the in vivo context: a metabolic game of give and take.EMBO reports · 2020Review
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Authors and funding
10 authors.
Funding
Abstract
backgroundColorectal cancer is a heterogeneous group of malignancies with complex molecular subtypes. While colon cancer has been widely investigated, studies on rectal cancer are very limited. Here, we performed multi-region whole-exome sequencing and single-cell whole-genome sequencing to examine the genomic intratumor heterogeneity (ITH) of rectal tumors.
methodsWe sequenced nine tumor regions and 88 single cells from two rectal cancer patients with tumors of the same molecular classification and characterized their mutation profiles and somatic copy number alterations (SCNAs) at the multi-region and the single-cell levels.
resultsA variable extent of genomic heterogeneity was observed between the two patients, and the degree of ITH increased when analyzed on the single-cell level. We found that major SCNAs were early events in cancer development and inherited steadily. Single-cell sequencing revealed mutations and SCNAs which were hidden in bulk sequencing. In summary, we studied the ITH of rectal cancer at regional and single-cell resolution and demonstrated that variable heterogeneity existed in two patients. The mutational scenarios and SCNA profiles of two patients with treatment naïve from the same molecular subtype are quite different.
conclusionsOur results suggest each tumor possesses its own architecture, which may result in different diagnosis, prognosis, and drug responses. Remarkable ITH exists in the two patients we have studied, providing a preliminary impression of ITH in rectal cancer.
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