ArticleBMC developmental biology2017
Tol2 transposon-mediated transgenesis in the Midas cichlid (Amphilophus citrinellus) - towards understanding gene function and regulatory evolution in an ecological model system for rapid phenotypic diversification.
Article in BMC developmental biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 14 citations in OpenAlex.
- TransTag enables simple and efficient transgene mapping in zebrafish via tagmentation.Cell reports methods · 2025Article
- Genome editing in East African cichlids and tilapias: state-of-the-art and future directions.Open biology · 2023Review
- East African cichlid fishes.EvoDevo · 2023Review
- Manipulation of the Tyrosinase gene permits improved CRISPR/Cas editing and neural imaging in cichlid fish.Scientific reports · 2021Article
- Developmental and Cellular Basis of Vertical Bar Color Patterns in the East African Cichlid FishFrontiers in cell and developmental biology · 2020Article
- Stable transgenesis in Astyanax mexicanus using the Tol2 transposase system.Developmental dynamics : an official publication of the American Association of Anatomists · 2019Article
- Searching for Sympatric Speciation in the Genomic Era.BioEssays : news and reviews in molecular, cellular and developmental biology · 2019Review
- Comprehensive Experimental System for a Promising Model Organism Candidate for Marine Teleosts.Scientific reports · 2019Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
backgroundThe Midas cichlid species complex (Amphilophus spp.) is widely known among evolutionary biologists as a model system for sympatric speciation and adaptive phenotypic divergence within extremely short periods of time (a few hundred generations). The repeated parallel evolution of adaptive phenotypes in this radiation, combined with their near genetic identity, makes them an excellent model for studying phenotypic diversification. While many ecological and evolutionary studies have been performed on Midas cichlids, the molecular basis of specific phenotypes, particularly adaptations, and their underlying coding and cis-regulatory changes have not yet been studied thoroughly.
resultsFor the first time in any New World cichlid, we use Tol2 transposon-mediated transgenesis in the Midas cichlid (Amphilophus citrinellus). By adapting existing microinjection protocols, we established an effective protocol for transgenesis in Midas cichlids. Embryos were injected with a Tol2 plasmid construct that drives enhanced green fluorescent protein (eGFP) expression under the control of the ubiquitin promoter. The transgene was successfully integrated into the germline, driving strong ubiquitous expression of eGFP in the first transgenic Midas cichlid line. Additionally, we show transient expression of two further transgenic constructs, ubiquitin::tdTomato and mitfa::eGFP. Transgenesis in Midas cichlids will facilitate further investigation of the genetic basis of species-specific traits, many of which are adaptations.
conclusionTransgenesis is a versatile tool not only for studying regulatory elements such as promoters and enhancers, but also for testing gene function through overexpression of allelic gene variants. As such, it is an important first step in establishing the Midas cichlid as a powerful model for studying adaptive coding and non-coding changes in an ecological and evolutionary context.
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