Evidence map›Paper›PMID 29169284›Full record

ArticleCell cycle (Georgetown, Tex.)2018

VEGFC/VEGFR3 Signaling Regulates Mouse Spermatogonial Cell Proliferation via the Activation of AKT/MAPK and Cyclin D1 Pathway and Mediates the Apoptosis by affecting Caspase 3/9 and Bcl-2.

Liangyu Zhao, Zijue Zhu, Chencheng Yao, Yuhua Huang, Erlei Zhi, Huixing Chen, Ruhui Tian, Peng Li, Qingqing Yuan, Yunjing Xue and 5 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell cycle (Georgetown, Tex.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
6.1field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 51 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. The lncRNA Gm8097 is associated with hypospermatogenesis.Clinical and experimental reproductive medicine · 2024
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. Article
  12. Article
  13. Article
  14. Review
  15. Article
  16. Article
  17. Article
  18. Article
  19. Correlation amongTranslational cancer research · 2020
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 2 institutions in 1 country.

Liangyu Zhaoa Department of Andrology, Center for Men's Health, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine , Shanghai Jiao Tong University, School of Medicine , Shanghai , China.
Zijue Zhua Department of Andrology, Center for Men's Health, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine , Shanghai Jiao Tong University, School of Medicine , Shanghai , China.
Chencheng Yaoa Department of Andrology, Center for Men's Health, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine , Shanghai Jiao Tong University, School of Medicine , Shanghai , China.
Yuhua Huanga Department of Andrology, Center for Men's Health, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine , Shanghai Jiao Tong University, School of Medicine , Shanghai , China.
Erlei Zhia Department of Andrology, Center for Men's Health, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine , Shanghai Jiao Tong University, School of Medicine , Shanghai , China.
Huixing Chena Department of Andrology, Center for Men's Health, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine , Shanghai Jiao Tong University, School of Medicine , Shanghai , China.
Ruhui Tiana Department of Andrology, Center for Men's Health, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine , Shanghai Jiao Tong University, School of Medicine , Shanghai , China.
Peng Lia Department of Andrology, Center for Men's Health, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine , Shanghai Jiao Tong University, School of Medicine , Shanghai , China.
Qingqing Yuanb State Key Laboratory of Oncogenes and Related Genes, Renji- Med X Clinical Stem Cell Research Center, Ren Ji Hospital , School of Medicine, Shanghai Jiao Tong University , Shanghai , China.
Yunjing Xuea Department of Andrology, Center for Men's Health, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine , Shanghai Jiao Tong University, School of Medicine , Shanghai , China.
Zhong Wana Department of Andrology, Center for Men's Health, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine , Shanghai Jiao Tong University, School of Medicine , Shanghai , China.
Chao Yanga Department of Andrology, Center for Men's Health, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine , Shanghai Jiao Tong University, School of Medicine , Shanghai , China.
Yuehua Gonga Department of Andrology, Center for Men's Health, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine , Shanghai Jiao Tong University, School of Medicine , Shanghai , China.
Zuping Heb State Key Laboratory of Oncogenes and Related Genes, Renji- Med X Clinical Stem Cell Research Center, Ren Ji Hospital , School of Medicine, Shanghai Jiao Tong University , Shanghai , China.
Zheng Lia Department of Andrology, Center for Men's Health, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine , Shanghai Jiao Tong University, School of Medicine , Shanghai , China.
Shanghai Jiao Tong University · CNShanghai First People's Hospital · CN

Funding

Alcohol dependence and brain endocannabinoid functionR01AA020404 · NIAAA · SCRIPPS RESEARCH INSTITUTE, THE · PI MARTIN-FARDON, REMI · 2011 to 2015
$2.2M
NIAAA NIH HHS R01 AA020404
6 · The paper itself

Abstract

We have previously shown that the transcript levels of Vegfc and its receptor Vegfr3 were high in spermatogonia and extremely low in spermatocytes and spermatids. However, it remains unknown about the functions and the mechanisms of VEGFC/VEGFR3 signaling in regulating the fate determinations of spermatogonia. To this end, here we explored the role and signaling pathways of VEGFC/VEGFR3 by using a cell line derived from immortalized mouse spermatogonia retaining markers of mitotic germ cells, namely GC-1 cells. VEGFR3 was expressed in mouse primary spermatogonia and GC-1 cells. VEGFC stimulated the proliferation and DNA synthesis of GC-1 cells and enhanced the phosphorylation of PI3K-AKT and MAPK, whereas LY294002 (an inhibitor for AKT) and CI-1040 (an inhibitor for MAPK) blocked the effect of VEGFC on GC-1 cell proliferation. Furthermore, VEGFC increased the transcripts of c-fos and Egr1 and protein levels of cyclin D1, PCNA and Bcl-2. Conversely, the blocking of VEGFC/VEGFR3 signaling by VEGFR3 knockdown reduced the phosphorylation of AKT/MAPK and decreased the levels of cyclin D1 and PCNA. Additionally, VEGFR3 knockdown not only resulted in more apoptosis of GC-1 cells but also led to a decrease of Bcl-2 and promoted the cleavage of Caspase-3/9 and PARP. Collectively, these data suggested that VEGFC/VEGFR3 signaling promotes the proliferation of GC-1 cells via the AKT /MAPK and cyclin D1 pathway and it inhibits the cell apoptosis through Caspase-3/9, PARP and Bcl-2. Thus, this study sheds a novel insight to the molecular mechanisms underlying the fate decisions of mammalian spermatogonia.

Indexed as

Cell ProliferationSignal TransductionAnimalsApoptosisCaspase 3Caspase 9Cell LineCyclin D1Early Growth Response Protein 1Extracellular Signal-Regulated MAP KinasesIndolesMaleMiceMice, Inbred ICRNaphthalenesPhosphatidylinositol 3-Kinase3-(4-dimethylamino-naphthalen-1-ylmethylene)-1,3-dihydro-indol-2-oneBcl2 protein, mouseCaspase 3Caspase 9Ccnd1 protein, mouseCyclin D1Early Growth Response Protein 1Egr1 protein, mouseExtracellular Signal-Regulated MAP KinasesIndolesNaphthalenesPhosphatidylinositol 3-KinaseProto-Oncogene Proteins c-aktProto-Oncogene Proteins c-bcl-2Proto-Oncogene Proteins c-fosVascular Endothelial Growth Factor Cvascular endothelial growth factor C, mouseVascular Endothelial Growth Factor Receptor-3AKTapoptosisGC-1 cellsMAPKproliferationVEGFC/VEGFR3

Identifiers

PMID29169284
PMCPMC5884123
OpenAlexW2769188981

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.