ArticleThe New phytologist2018
N-terminomics reveals control of Arabidopsis seed storage proteins and proteases by the Arg/N-end rule pathway.
Article in The New phytologist, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 55 citations in OpenAlex.
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- Ubiquitin E3 ligases in the plant Arg/N-degron pathway.The Biochemical journal · 2024Review
- Hypoxia represses pattern-triggered immune responses in Arabidopsis.Plant physiology · 2024Article
- BIG enhances Arg/N-degron pathway-mediated protein degradation to regulate Arabidopsis hypoxia responses and suberin deposition.The Plant cell · 2024Article
- The proteome of Nicotiana benthamiana is shaped by extensive protein processing.The New phytologist · 2024Article
- Ethylene augments root hypoxia tolerance via growth cessation and reactive oxygen species amelioration.Plant physiology · 2022Article
- Label-Free Quantitative Proteomics Reveal the Involvement of PRT6 inInternational journal of molecular sciences · 2022Article
- Protein Processing in Plant Mitochondria Compared to Yeast and Mammals.Frontiers in plant science · 2022Review
- Nitric oxide function during oxygen deprivation in physiological and stress processes.Journal of experimental botany · 2021Review
- To New Beginnings: Riboproteogenomics Discovery of N-Terminal Proteoforms inFrontiers in plant science · 2021Article
- A simple and efficientPlant direct · 2020Article
- Unwinding BRAHMA Functions in Plants.Genes · 2020Review
- ThePlant direct · 2019Article
- Ethylene-mediated nitric oxide depletion pre-adapts plants to hypoxia stress.Nature communications · 2019Article
- Nitric oxide molecular targets: reprogramming plant development upon stress.Journal of experimental botany · 2019Review
- Differential N-end Rule Degradation of RIN4/NOI Fragments Generated by the AvrRpt2 Effector Protease.Plant physiology · 2019Article
- Tandem Fluorescent Protein Timers for Noninvasive Relative Protein Lifetime Measurement in Plants.Plant physiology · 2019Article
- Plant proteases during developmental programmed cell death.Journal of experimental botany · 2019Review
- New beginnings and new ends: methods for large-scale characterization of protein termini and their use in plant biology.Journal of experimental botany · 2019Review
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9 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The N-end rule pathway of targeted protein degradation is an important regulator of diverse processes in plants but detailed knowledge regarding its influence on the proteome is lacking. To investigate the impact of the Arg/N-end rule pathway on the proteome of etiolated seedlings, we used terminal amine isotopic labelling of substrates with tandem mass tags (TMT-TAILS) for relative quantification of N-terminal peptides in prt6, an Arabidopsis thaliana N-end rule mutant lacking the E3 ligase PROTEOLYSIS6 (PRT6). TMT-TAILS identified over 4000 unique N-terminal peptides representing c. 2000 protein groups. Forty-five protein groups exhibited significantly increased N-terminal peptide abundance in prt6 seedlings, including cruciferins, major seed storage proteins, which were regulated by Group VII Ethylene Response Factor (ERFVII) transcription factors, known substrates of PRT6. Mobilisation of endosperm α-cruciferin was delayed in prt6 seedlings. N-termini of several proteases were downregulated in prt6, including RD21A. RD21A transcript, protein and activity levels were downregulated in a largely ERFVII-dependent manner. By contrast, cathepsin B3 protein and activity were upregulated by ERFVIIs independent of transcript. We propose that the PRT6 branch of the pathway regulates protease activities in a complex manner and optimises storage reserve mobilisation in the transition from seed to seedling via control of ERFVII action.
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