ArticleNPJ microgravity2017
Successful amplification of DNA aboard the International Space Station.
Article in NPJ microgravity, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 49 citations in OpenAlex.
- Pharmaceutical and biomedical challenges for crew autonomy in health preservation during future exploration missions.Communications medicine · 2025Review
- Validation of Cell-Free Protein Synthesis Aboard the International Space Station.ACS synthetic biology · 2024Article
- A fluorescence viewer for rapid molecular assay readout in space and low-resource terrestrial environments.PloS one · 2024Article
- The Development of a 3D Printer-Inspired, Microgravity-Compatible Sample Preparation Device for Future Use Inside the International Space Station.Micromachines · 2023Article
- Space Medicines for Space Health.ACS medicinal chemistry letters · 2022Article
- Picroscope: low-cost system for simultaneous longitudinal biological imaging.Communications biology · 2021Article
- A miniPCR-Duplex Lateral Flow Dipstick Platform for Rapid and Visual Diagnosis of Lymphatic Filariae Infection.Diagnostics (Basel, Switzerland) · 2021Article
- PCR-assisted impedimetric biosensor for colibactin-encoding pks genomic island detection in E. coli samples.Analytical and bioanalytical chemistry · 2021Article
- A Scalable, Easy-to-Deploy Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material.Journal of clinical microbiology · 2021Article
- Article
- A CRISPR-based assay for the study of eukaryotic DNA repair onboard the International Space Station.PloS one · 2021Article
- Nucleic acid detection aboard the International Space Station by colorimetric loop-mediated isothermal amplification (LAMP).FASEB bioAdvances · 2020Article
- Validating an Automated Nucleic Acid Extraction Device forFrontiers in microbiology · 2020Article
- Article
- Characterization of the total and viable bacterial and fungal communities associated with the International Space Station surfaces.Microbiome · 2019Article
- Dietary intervention of mice using an improved Multiple Artificial-gravity Research System (MARS) under artificial 1NPJ microgravity · 2019Article
- Article
- "The Smartphone's Guide to the Galaxy": In Situ Analysis in Space.Biosensors · 2018Review
- Water Quality and Total Microbial Load: A Double-Threshold Identification Procedure Intended for Space Applications.Frontiers in microbiology · 2018Article
- Gene expression studies using a miniaturized thermal cycler system on board the International Space Station.PloS one · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
13 authors at 5 institutions in 2 countries.
Funding
Abstract
As the range and duration of human ventures into space increase, it becomes imperative that we understand the effects of the cosmic environment on astronaut health. Molecular technologies now widely used in research and medicine will need to become available in space to ensure appropriate care of astronauts. The polymerase chain reaction (PCR) is the gold standard for DNA analysis, yet its potential for use on-orbit remains under-explored. We describe DNA amplification aboard the International Space Station (ISS) through the use of a miniaturized miniPCR system. Target sequences in plasmid, zebrafish genomic DNA, and bisulfite-treated DNA were successfully amplified under a variety of conditions. Methylation-specific primers differentially amplified bisulfite-treated samples as would be expected under standard laboratory conditions. Our findings establish proof of concept for targeted detection of DNA sequences during spaceflight and lay a foundation for future uses ranging from environmental monitoring to on-orbit diagnostics.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.