ArticleScientific reports2017
Influence of experimental parameters on the laser heating of an optical trap.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Single Staphylococcus aureus SauUSI restriction enzyme motors fragment methylated DNA.Nature chemical biology · 2026Article
- Obstacles Regulate Membrane Tension Propagation to Enable Localized Mechanotransduction.bioRxiv : the preprint server for biology · 2025Article
- Measuring age-dependent viscoelasticity of organelles, cells and organisms with time-shared optical tweezer microrheology.Nature nanotechnology · 2025Article
- Optical tweezers in biomedical research - progress and techniques.Journal of medicine and life · 2024Review
- The bacterial flagellum as an object for optical trapping.Biophysical reviews · 2024Review
- Shining Light in Mechanobiology: Optical Tweezers, Scissors, and Beyond.ACS photonics · 2024Review
- Article
- Looking at Biomolecular Interactions through the Lens of Correlated Fluorescence Microscopy and Optical Tweezers.International journal of molecular sciences · 2023Review
- Thermal fluctuations of the lipid membrane determine particle uptake into Giant Unilamellar Vesicles.Nature communications · 2023Article
- Optical tweezers across scales in cell biology.Trends in cell biology · 2022Review
- Foregut organ progenitors and their niche display distinct viscoelastic properties in vivo during early morphogenesis stages.Communications biology · 2022Article
- Intense femtosecond optical pulse shaping approaches to spatiotemporal control.Frontiers in chemistry · 2022Article
- Detachment ofSensors (Basel, Switzerland) · 2020Article
- Manipulating rod-shaped bacteria with optical tweezers.Scientific reports · 2019Article
- Optical Tweezers: Phototoxicity and Thermal Stress in Cells and Biomolecules.Micromachines · 2019Review
- Effects of near infrared focused laser on the fluorescence of labelled cell membrane.Scientific reports · 2018Article
- Controlled Mechanical Motions of Microparticles in Optical Tweezers.Micromachines · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In optical tweezers, heating of the sample due to absorption of the laser light is a major concern as temperature plays an important role at microscopic scale. A popular rule of thumb is to consider that, at the typical wavelength of 1064 nm, the focused laser induces a heating rate of B = 1 °C/100 mW. We analysed this effect under different routine experimental conditions and found a remarkable variability in the temperature increase. Importantly, we determined that temperature can easily rise by as much as 4 °C at a relatively low power of 100 mW, for dielectric, non-absorbing particles with certain sets of specific, but common, parameters. Heating was determined from measurements of light momentum changes under drag forces at different powers, which proved to provide precise and robust results in watery buffers. We contrasted the experiments with computer simulations and obtained good agreement. These results suggest that this remarkable heating could be responsible for changes in the sample under study and could lead to serious damage of live specimens. It is therefore advisable to determine the temperature increase in each specific experiment and avoid the use of a universal rule that could inadvertently lead to critical changes in the sample.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.