ArticleBMC pulmonary medicine2017
Detection of pathogens by real-time PCR in adult patients with acute exacerbation of bronchial asthma.
Article in BMC pulmonary medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 2 of them syntheses that pooled it.
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Who cites it
16 citing papers in PubMed, 2 syntheses or guidelines pooled it, 26 citations in OpenAlex.
- Prevalence of respiratory viruses in stable and acute asthma: a systematic review and meta-analysis.European respiratory review : an official journal of the European Respiratory Society · 2026Pooled it
- Comparison of detection rate of 16 sampling methods for respiratory viruses: a Bayesian network meta-analysis of clinical data and systematic review.BMJ global health · 2020Pooled it
- Bioassays in Allergy: Technical Characteristics, Limitations, and Pathways to Clinical Implementation-An EAACI Position Paper.Clinical and translational allergy · 2026Review
- Article
- The Dual Role of the Airway Epithelium in Asthma: Active Barrier and Regulator of Inflammation.Cells · 2023Review
- Article
- Nicoletella semolina in the airways of healthy horses and horses with severe asthma.Journal of veterinary internal medicine · 2021Article
- Interrupting the Conversation: Implications for Crosstalk Between Viral and Bacterial Infections in the Asthmatic Airway.Frontiers in allergy · 2021Review
- Viruses of Respiratory Tract: an Observational Retrospective Study on Hospitalized Patients in Rome, Italy.Microorganisms · 2020Article
- Tracheal microbial populations in horses with moderate asthma.Journal of veterinary internal medicine · 2020Article
- Asthma and/or hay fever as predictors of fertility/impaired fecundity in U.S. women: National Survey of Family Growth.Scientific reports · 2019Article
- The Impact of Ambient Environmental Exposures to Microbial Products on Asthma Outcomes from Birth to Childhood.Current allergy and asthma reports · 2019Review
- Viral and Bacterial Etiology of Acute Febrile Respiratory Syndrome among Patients in Qinghai, China.Biomedical and environmental sciences : BES · 2019Article
- Impact of eosinophil-peroxidase (EPX) deficiency on eosinophil structure and function in mouse airways.Journal of leukocyte biology · 2019Article
- Laboratory epidemiology of respiratory viruses in a large children's hospital: A STROBE-compliant article.Medicine · 2018Observational
- Prevalence of acute infection in adults with asthma exacerbation: A systematic review and meta-analysis.Annals of thoracic medicineArticle
Corrections and comments
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Authors and funding
16 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRespiratory tract infection is a major cause of acute exacerbation of bronchial asthma (AEBA). Although recent findings suggest that common bacteria are causally associated with AEBA, a comprehensive epidemiologic analysis of infectious pathogens including common/atypical bacteria and viruses in AEBA has not been performed. Accordingly, we attempted to detect pathogens during AEBA by using real-time polymerase chain reaction (PCR) in comparison to conventional methods.
methodsWe prospectively enroled adult patients with AEBA from August 2012 to March 2014. Infectious pathogens collected in nasopharyngeal swab and sputum samples were examined in each patient by conventional methods and real-time PCR, which can detect 6 bacterial and 11 viral pathogens. The causal association of these pathogens with AEBA severity and their frequency of monthly distribution were also examined.
resultsAmong the 64 enroled patients, infectious pathogens were detected in 49 patients (76.6%) using real-time PCR and in 14 patients (21.9%) using conventional methods (p < 0.001). Real-time PCR detected bacteria in 29 patients (45.3%) and respiratory viruses in 28 patients (43.8%). Haemophilus influenzae was the most frequently detected microorganism (26.6%), followed by rhinovirus (15.6%). Influenza virus was the significant pathogen associated with severe AEBA. Moreover, AEBA occurred most frequently during November to January.
conclusionsReal-time PCR was more useful than conventional methods to detect infectious pathogens in patients with AEBA. Accurate detection of pathogens with real-time PCR may enable the selection of appropriate anti-bacterial/viral agents as a part of the treatment for AEBA.
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