ArticlePloS one2017
Age-related differences in the respiratory microbiota of chickens.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Exploring the chicken respiratory microbiome: a comprehensive meta-analysis of factors shaping chicken respiratory microbiota.Scientific reports · 2026Pooled it
- Respiratory microbiota maturation enables machine learning based age prediction in chickens.BMC microbiology · 2026Article
- Breaking the Cycle: A Yeast Mannan-Rich Fraction Beneficially Modulates Egg Quality and the Antimicrobial Resistome Associated with Layer Hen Caecal Microbiomes under Commercial Conditions.Microorganisms · 2024Article
- Exploring the complexities of poultry respiratory microbiota: colonization, composition, and impact on health.Animal microbiome · 2024Review
- Bacterial communities along parrot digestive and respiratory tracts: the effects of sample type, species and time.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- A comprehensive longitudinal study of gut microbiota dynamic changes in laying hens at four growth stages prior to egg production.Animal bioscience · 2023Article
- Alteration of the Chicken Upper Respiratory Microbiota, Following H9N2 Avian Influenza Virus Infection.Pathogens (Basel, Switzerland) · 2023Article
- A succession of pulmonary microbiota in broilers during the growth cycle.Poultry science · 2023Article
- The Microbial Community of the Respiratory Tract of Commercial Chickens and Turkeys.Microorganisms · 2022Article
- Rational Use of Danofloxacin for Treatment ofAntibiotics (Basel, Switzerland) · 2022Article
- Metagenomics analysis of the fecal microbiota in Ring-necked pheasants (Saudi journal of biological sciences · 2022Article
- Uncovering the core principles of the gut-lung axis to enhance innate immunity in the chicken.Frontiers in immunology · 2022Article
- Low pathogenic avian influenza virus infection retards colon microbiota diversification in two different chicken lines.Animal microbiome · 2021Article
- Metagenomic Analysis of the Respiratory Microbiome of a Broiler Flock from Hatching to Processing.Microorganisms · 2021Article
- Bacterial communities of the upper respiratory tract of turkeys.Scientific reports · 2021Article
- Assessment of two DNA extraction kits for profiling poultry respiratory microbiota from multiple sample types.PloS one · 2021Article
- Prudent Use of Tylosin for Treatment ofFrontiers in microbiology · 2021Article
- Dynamic Changes in Lung Microbiota of Broilers in Response to Aging and Ammonia Stress.Frontiers in microbiology · 2021Article
- The Airway Pathobiome in Complex Respiratory Diseases: A Perspective in Domestic Animals.Frontiers in cellular and infection microbiology · 2021Review
- Respiratory and Gut Microbiota in Commercial Turkey Flocks with Disparate Weight Gain Trajectories Display Differential Compositional Dynamics.Applied and environmental microbiology · 2020Article
Corrections and comments
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3 authors.
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Abstract
In this era of next generation sequencing technologies it is now possible to characterise the chicken respiratory microbiota without the biases inherent to traditional culturing techniques. However, little research has been performed in this area. In this study we characterise and compare buccal, nasal and lung microbiota samples from chickens in three different age groups using 16S rRNA gene analysis. Buccal and nasal swabs were taken from birds aged 2 days (n = 5), 3 weeks (n = 5) and 30 months (n = 6). Bronchoalveolar lavage (BAL) samples were also collected alongside reagent only controls. DNA was extracted from these samples and the V2-V3 region of the 16S rRNA gene was amplified and sequenced. Quality control and OTU clustering were performed in mothur. Bacterial DNA was quantified using qPCR, amplifying the V3 region of the 16S rRNA gene. We found significant differences between the quantity and types of bacteria sampled at the three different respiratory sites. We also found significant differences in the composition, richness and diversity of the bacterial communities in buccal, nasal and BAL fluid samples between age groups. We identified several bacteria which had previously been isolated from the chicken respiratory tract in culture based studies, including lactobacilli and staphylococci. However, we also identified bacteria which have not previously been cultured from the respiratory tract of the healthy chicken. We conclude that our study can be used as a baseline that future chicken respiratory microbiota studies can build upon.
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