ArticleACS nano2017
Structural Characterization of Native and Modified Encapsulins as Nanoplatforms for in Vitro Catalysis and Cellular Uptake.
Article in ACS nano, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
44 citing papers in PubMed.
- Encapsulin-Protected Immunotherapeutic Complexes: Bacteria-Derived Nanoparticles for mRNA Delivery to Eukaryotic Cells.ChemMedChem · 2026Article
- Self-assembling protein cages: from coiled-coil module to machine learning-drivenMaterials advances · 2026Review
- Innovation in mRNA Vaccines and RNAi via Protein Nanocages.Vaccines · 2025Review
- High-Fidelity In Vitro Packaging of Diverse Synthetic Cargo into Encapsulin Protein Cages.Angewandte Chemie (International ed. in English) · 2025Article
- Review
- Surface Engineering of the Encapsulin Nanocompartment ofACS omega · 2025Article
- In situ and in vitro cryo-EM reveal structures of mycobacterial encapsulin assembly intermediates.Communications biology · 2025Article
- Structural Characterization ofMicroorganisms · 2024Article
- Molecular Design of Encapsulin Protein Nanoparticles to Display Rotavirus Antigens for Enhancing Immunogenicity.Vaccines · 2024Article
- Encapsulation of Transketolase intoACS applied bio materials · 2024Article
- Dye-Decolorizing Peroxidase ofACS omega · 2024Article
- Internalization of transferrin-taggedExperimental biology and medicine (Maywood, N.J.) · 2024Article
- Genetically encoded multimeric tags for subcellular protein localization in cryo-EM.Nature methods · 2023Article
- Encapsulin cargo loading: progress and potential.Journal of materials chemistry. B · 2023Review
- Exploring the Extreme Acid Tolerance of a Dynamic Protein Nanocage.Biomacromolecules · 2023Article
- Charge Transport across Proteins inside Proteins: Tunneling across Encapsulin Protein Cages and the Effect of Cargo Proteins.Biomolecules · 2023Article
- Encapsulins: Structure, Properties, and Biotechnological Applications.Biochemistry. Biokhimiia · 2023Review
- Article
- Encapsulins.Annual review of biochemistry · 2022Review
- Bacterial Nanocompartments: Structures, Functions, and Applications.Journal of bacteriology · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent years have witnessed the emergence of bacterial semiorganelle encapsulins as promising platforms for bio-nanotechnology. To advance the development of encapsulins as nanoplatforms, a functional and structural basis of these assemblies is required. Encapsulin from Brevibacterium linens is known to be a protein-based vessel for an enzyme cargo in its cavity, which could be replaced with a foreign cargo, resulting in a modified encapsulin. Here, we characterize the native structure of B. linens encapsulins with both native and foreign cargo using cryo-electron microscopy (cryo-EM). Furthermore, by harnessing the confined enzyme (i.e., a peroxidase), we demonstrate the functionality of the encapsulin for an in vitro surface-immobilized catalysis in a cascade pathway with an additional enzyme, glucose oxidase. We also demonstrate the in vivo functionality of the encapsulin for cellular uptake using mammalian macrophages. Unraveling both the structure and functionality of the encapsulins allows transforming biological nanocompartments into functional systems.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.