ArticleACS nano2017
Hybrid Structured Illumination Expansion Microscopy Reveals Microbial Cytoskeleton Organization.
Article in ACS nano, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
61 citing papers in PubMed.
- Nucleolar reorganization on stress depends on physicochemical changes due to reduced nascent rRNA synthesis.EMBO reports · 2026Article
- Characterization of membrane structures regulating primary ciliogenesis by quantitative isotropic ultrastructure imaging.Nature communications · 2026Article
- Article
- IntAct-U-ExM enables super-resolution imaging of isoform-specific actin networks across species.PLoS biology · 2026Article
- The cytoskeleton of pathogenic protists.Bioscience reports · 2026Review
- Expansion Microscopy reveals changes in cellular and extracellular structures between healthy and perturbed tendons.bioRxiv : the preprint server for biology · 2026Article
- 3D Expansion-PALM (PhotoActivated Localization Microscopy) Dissects Protein-Protein Interactions Down to the Molecular Scale in Bacteria.Microorganisms · 2026Article
- Review of expansion microscopy combined with advanced imaging modalities.Journal of microscopy · 2026Review
- Ultrastructure Expansion Microscopy for the Analysis of Trypanosoma cruzi Infection.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Advances in high-resolution imaging of centrioles and cilia.Journal of cell science · 2025Review
- DNA-PAINT Imaging with Hydrogel Imprinting and Clearing.ACS sensors · 2025Article
- Super-resolution expansion microscopy in plant roots.The Plant cell · 2025Article
- Visualisation ofLife science alliance · 2025Article
- Surpassing the Diffraction Limit in Label-Free Optical Microscopy.ACS photonics · 2024Review
- Stimuli-Responsive Phosphate Hydrogel: A Study on Swelling Behavior, Mechanical Properties, and Application in Expansion Microscopy.ACS omega · 2024Article
- Subdiffraction Imaging of Cleared and Expanded Large-Scale Tissues.Chemical & biomedical imaging · 2024Review
- Expanding boundaries - a cell biologist's guide to expansion microscopy.Journal of cell science · 2024Review
- Combining single-molecule and expansion microscopy in fission yeast to visualize protein structures at the nanostructural level.Open biology · 2024Article
- Expansion Microscopy of Ciliary Proteins.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Superresolution Expansion Microscopy in Dictyostelium Amoebae.Methods in molecular biology (Clifton, N.J.) · 2024Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Recently developed tissue-hydrogel methods for specimen expansion now enable researchers to perform super-resolution microscopy with ∼65 nm lateral resolution using ordinary microscopes, standard fluorescent probes, and inexpensive reagents. Here we use the combination of specimen expansion and the optical super-resolution microscopy technique structured illumination microscopy (SIM) to extend the spatial resolution to ∼30 nm. We apply this hybrid method, which we call ExSIM, to study the cytoskeleton of the important human pathogen Giardia lamblia including the adhesive disc and flagellar axonemes. We determined the localization of two recently identified disc-associated proteins, including DAP86676 , which localizes to disc microribbons, and the functionally unknown DAP16263 , which primarily localizes to dorsal microtubules of the disc overlap zone and the paraflagellar rod of ventral axonemes. Based on its strong performance in revealing known and unknown details of the ultrastructure of Giardia, we find that ExSIM is a simple, rapid, and powerful super-resolution method for the study of fixed specimens, and it should be broadly applicable to other biological systems of interest.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.