ArticleNucleic acids research2018
Development and application of a recombination-based library versus library high- throughput yeast two-hybrid (RLL-Y2H) screening system.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 52 citations in OpenAlex.
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- Interspecies rice versus Arabidopsis thaliana protein-protein interactome profiling by touch-down overlapping PCR coupled with HiFi long-read sequencing.Journal of integrative plant biology · 2026Article
- Transcription Factor PFB1 Is Required for the Botrytis cinerea Effector BcSCR1-Mediated Pathogenesis.Molecular plant pathology · 2026Article
- Mycobacterium tuberculosis uses intrinsically disordered, fast evolving proteins to interact with conserved host factors.Genome biology · 2025Article
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- Global Protein Interactome Mapping in Rice Using Barcode-Indexed PCR Coupled with HiFi Long-Read Sequencing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Structure-Based Modeling of the Gut Bacteria-Host Interactome Through Statistical Analysis of Domain-Domain Associations Using Machine Learning.Biotech (Basel (Switzerland)) · 2025Article
- Structure-Based Deep Learning Framework for Modeling Human-Gut Bacterial Protein Interactions.Proteomes · 2025Article
- High-Throughput Yeast Two-Hybrid Technology for Investigating Molecular Virology.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Massively parallel measurement of protein-protein interactions by sequencing using MP3-seq.Nature chemical biology · 2024Article
- Dissecting the molecular basis of spike traits by integrating gene regulatory networks and genetic variation in wheat.Plant communications · 2024Article
- Interactome between ASFV and host immune pathway proteins.mSystems · 2023Article
- A multiplexed bacterial two-hybrid for rapid characterization of protein-protein interactions and iterative protein design.Nature communications · 2023Article
- Next-Generation Yeast Two-Hybrid Screening to Discover Protein-Protein Interactions.Methods in molecular biology (Clifton, N.J.) · 2023Article
- ZmHOX32 is related to photosynthesis and likely functions in plant architecture of maize.Frontiers in plant science · 2023Article
- Article
- Barcode fusion genetics-protein-fragment complementation assay (BFG-PCA): tools and resources that expand the potential for binary protein interaction discovery.Nucleic acids research · 2022Article
- Article
- Unraveling Protein Interactions between the Temperate Virus Bam35 and ItsInternational journal of molecular sciences · 2021Article
- Revealing protein-protein interactions at the transcriptome scale by sequencing.Molecular cell · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
21 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein-protein interaction (PPI) network maintains proper function of all organisms. Simple high-throughput technologies are desperately needed to delineate the landscape of PPI networks. While recent state-of-the-art yeast two-hybrid (Y2H) systems improved screening efficiency, either individual colony isolation, library preparation arrays, gene barcoding or massive sequencing are still required. Here, we developed a recombination-based 'library vs library' Y2H system (RLL-Y2H), by which multi-library screening can be accomplished in a single pool without any individual treatment. This system is based on the phiC31 integrase-mediated integration between bait and prey plasmids. The integrated fragments were digested by MmeI and subjected to deep sequencing to decode the interaction matrix. We applied this system to decipher the trans-kingdom interactome between Mycobacterium tuberculosis and host cells and further identified Rv2427c interfering with the phagosome-lysosome fusion. This concept can also be applied to other systems to screen protein-RNA and protein-DNA interactions and delineate signaling landscape in cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.