ArticleSensors (Basel, Switzerland)2017
Dielectrophoretic Separation of Live and Dead Monocytes Using 3D Carbon-Electrodes.
Article in Sensors (Basel, Switzerland), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Comparing Different Light Models for Virtual Electrodes in Optoelectronic Tweezers.Electrophoresis · 2025Article
- Advances in Cell Separation: Harnessing DNA Nanomaterials for High-Specificity Recognition and Isolation.Chem & bio engineering · 2025Review
- Design and Fabrication of Microelectrodes for Dielectrophoresis and Electroosmosis in Microsystems for Bio-Applications.Micromachines · 2025Review
- Experimental and Numerical Studies of the Temperature Field in a Dielectrophoretic Cell Separation Device Subject to Joule Heating.Sensors (Basel, Switzerland) · 2024Article
- Microfluidic Blood Separation: Key Technologies and Critical Figures of Merit.Micromachines · 2023Review
- A High-Throughput Microfluidic Cell Sorter Using a Three-Dimensional Coupled Hydrodynamic-Dielectrophoretic Pre-Focusing Module.Micromachines · 2023Article
- Continuous Flow Separation of Live and Dead Cells Using Gravity Sedimentation.Micromachines · 2023Article
- New Generation Dielectrophoretic-Based Microfluidic Device for Multi-Type Cell Separation.Biosensors · 2023Article
- Microfluidic Systems for Blood and Blood Cell Characterization.Biosensors · 2022Review
- Rational Design and Numerical Analysis of a Hybrid Floating cIDE Separator for Continuous Dielectrophoretic Separation of Microparticles at High Throughput.Micromachines · 2022Article
- Integration of a Dielectrophoretic Tapered Aluminum Microelectrode Array with a Flow Focusing Technique.Sensors (Basel, Switzerland) · 2021Article
- Methods of Generating Dielectrophoretic Force for Microfluidic Manipulation of Bioparticles.ACS biomaterials science & engineering · 2021Review
- Dynamically controlled dielectrophoresis using resonant tuning.Electrophoresis · 2021Article
- Characterization and Separation of Live and Dead Yeast Cells Using CMOS-Based DEP Microfluidics.Micromachines · 2021Article
- Microscale nonlinear electrokinetics for the analysis of cellular materials in clinical applications: a review.Mikrochimica acta · 2021Review
- Quantifying Heterogeneity According to Deformation of the U937 Monocytes and U937-Differentiated Macrophages Using 3D Carbon Dielectrophoresis in Microfluidics.Micromachines · 2020Article
- Dielectrophoretic Immobilization of Yeast Cells Using CMOS Integrated Microfluidics.Micromachines · 2020Article
- Facile template-free synthesis of multifunctional 3D cellular carbon from edible rice paper.RSC advances · 2020Article
- Characterization of the Dielectrophoretic Response of Different Candida Strains Using 3D Carbon Microelectrodes.Micromachines · 2020Article
- Glassy Carbon: A Promising Material for Micro- and Nanomanufacturing.Materials (Basel, Switzerland) · 2018Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Blood has been the most reliable body fluid commonly used for the diagnosis of diseases. Although there have been promising investigations for the development of novel lab-on-a-chip devices to utilize other body fluids such as urine and sweat samples in diagnosis, their stability remains a problem that limits the reliability and accuracy of readouts. Hence, accurate and quantitative separation and characterization of blood cells are still crucial. The first step in achieving high-resolution characteristics for specific cell subpopulations from the whole blood is the isolation of pure cell populations from a mixture of cell suspensions. Second, live cells need to be purified from dead cells; otherwise, dead cells might introduce biases in the measurements. In addition, the separation and characterization methods being used must preserve the genetic and phenotypic properties of the cells. Among the characterization and separation approaches, dielectrophoresis (DEP) is one of the oldest and most efficient label-free quantification methods, which directly purifies and characterizes cells using their intrinsic, physical properties. In this study, we present the dielectrophoretic separation and characterization of live and dead monocytes using 3D carbon-electrodes. Our approach successfully removed the dead monocytes while preserving the viability of the live monocytes. Therefore, when blood analyses and disease diagnosis are performed with enriched, live monocyte populations, this approach will reduce the dead-cell contamination risk and achieve more reliable and accurate test results.
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