ArticleMicrobiologyOpen2018
A transposon mutant library of Bacillus cereus ATCC 10987 reveals novel genes required for biofilm formation and implicates motility as an important factor for pellicle-biofilm formation.
Article in MicrobiologyOpen, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Bacillus cereus PelAThe Journal of biological chemistry · 2026Article
- Exploring the antibacterial mechanism and biofilm inhibition ofCurrent research in food science · 2026Article
- ARTP mutagenesis for genome-wide identification of genes important for biofilm regulation in spoilage bacteriumApplied and environmental microbiology · 2025Article
- Genomic basis and functional characterization of the exopolysaccharide production by a thermotolerantFrontiers in microbiology · 2025Article
- A rare waterborne outbreak ofEmerging microbes & infections · 2024Article
- Sliding motility of Bacillus cereus mediates vancomycin pseudo-resistance during antimicrobial susceptibility testing.The Journal of antimicrobial chemotherapy · 2024Article
- Investigating the Role of OrbF in Biofilm Biosynthesis and Regulation of Biofilm-Associated Genes inFoods (Basel, Switzerland) · 2024Article
- Seed-Borne Bacterial Diversity of Fescue (Microorganisms · 2024Article
- Extracellular Interaction ofViruses · 2023Article
- Review
- Characterization of strain-specific Bacillus cereus swimming motility and flagella by means of specific antibodies.PloS one · 2022Article
- Transcriptomic and Phenomic Investigations Reveal Elements in Biofilm Repression and Formation in the CyanobacteriumFrontiers in microbiology · 2022Article
- Adaptation of Bacillus thuringiensis to Plant Colonization Affects Differentiation and Toxicity.mSystems · 2021Article
- Novel Genes Required for Surface-Associated Motility in Acinetobacter baumannii.Current microbiology · 2021Article
- An Overview of Biological and Computational Methods for Designing Mechanism-Informed Anti-biofilm Agents.Frontiers in microbiology · 2021Review
- TheToxins · 2020Review
- Biofilm Production by Enterotoxigenic Strains ofMicroorganisms · 2020Article
- Discovery and characterization of a Gram-positive Pel polysaccharide biosynthetic gene cluster.PLoS pathogens · 2020Article
- Two genomic regions encoding exopolysaccharide production systems have complementary functions in B. cereus multicellularity and host interaction.Scientific reports · 2020Article
- MogR Is a Ubiquitous Transcriptional Repressor Affecting Motility, Biofilm Formation and Virulence inFrontiers in microbiology · 2020Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus cereus is one of the most common opportunistic pathogens causing foodborne illness, as well as a common source of contamination in the dairy industry. B. cereus can form robust biofilms on food processing surfaces, resulting in food contamination due to shedding of cells and spores. Despite the medical and industrial relevance of this species, the genetic basis of biofilm formation in B. cereus is not well studied. In order to identify genes required for biofilm formation in this bacterium, we created a library of 5000 + transposon mutants of the biofilm-forming strain B. cereusATCC 10987, using an unbiased mariner transposon approach. The mutant library was screened for the ability to form a pellicle biofilm at the air-media interface, as well as a submerged biofilm at the solid-media interface. A total of 91 genes were identified as essential for biofilm formation. These genes encode functions such as chemotaxis, amino acid metabolism and cellular repair mechanisms, and include numerous genes not previously known to be required for biofilm formation. Although the majority of disrupted genes are not directly responsible for motility, further investigations revealed that the vast majority of the biofilm-deficient mutants were also motility impaired. This observation implicates motility as a pivotal factor in the formation of a biofilm by B. cereus. These results expand our knowledge of the fundamental molecular mechanisms of biofilm formation by B. cereus.
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