Evidence map›Paper›PMID 29164416›Full record

ArticleBiotechnology letters2018

High-throughput and direct measurement of androgen levels using turbulent flow chromatography liquid chromatography-triple quadrupole mass spectrometry (TFC-LC-TQMS) to discover chemicals that modulate dihydrotestosterone production in human prostate cancer cells.

Kyungsu Kang, Lei Peng, Yu-Jin Jung, Joo Yeon Kim, Eun Ha Lee, Hee Ju Lee, Sang Min Kim, Sang Hyun Sung, Cheol-Ho Pan, Yongsoo Choi

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology letters, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Kyungsu KangSystems Biotechnology Research Center, Korea Institute of Science and Technology, Gangneung, 25451, Republic of Korea.
Lei PengSystems Biotechnology Research Center, Korea Institute of Science and Technology, Gangneung, 25451, Republic of Korea.
Yu-Jin JungSystems Biotechnology Research Center, Korea Institute of Science and Technology, Gangneung, 25451, Republic of Korea.
Joo Yeon KimSystems Biotechnology Research Center, Korea Institute of Science and Technology, Gangneung, 25451, Republic of Korea.
Eun Ha LeeSystems Biotechnology Research Center, Korea Institute of Science and Technology, Gangneung, 25451, Republic of Korea.
Hee Ju LeeSystems Biotechnology Research Center, Korea Institute of Science and Technology, Gangneung, 25451, Republic of Korea.
Sang Min KimSystems Biotechnology Research Center, Korea Institute of Science and Technology, Gangneung, 25451, Republic of Korea.
Sang Hyun SungCollege of Pharmacy and Research, Institute of Pharmaceutical Sciences, Seoul National University, Seoul, 08826, Republic of Korea.
Cheol-Ho PanSystems Biotechnology Research Center, Korea Institute of Science and Technology, Gangneung, 25451, Republic of Korea.
Yongsoo ChoiSystems Biotechnology Research Center, Korea Institute of Science and Technology, Gangneung, 25451, Republic of Korea. yongsoo.choi@kist.re.kr.
Korea Institute of Science and Technology · KRKorea University of Science and Technology · KRSeoul National University · KR

Funding

Korea Institute of Science and Technology 2Z04930Rural Development Administration PJ01129501
6 · The paper itself

Abstract

objectivesTo develop a high-throughput screening system to measure the conversion of testosterone to dihydrotestosterone (DHT) in cultured human prostate cancer cells using turbulent flow chromatography liquid chromatography-triple quadrupole mass spectrometry (TFC-LC-TQMS).

resultsAfter optimizing the cell reaction system, this method demonstrated a screening capability of 103 samples, including 78 single compounds and 25 extracts, in less than 12 h without manual sample preparation. Consequently, fucoxanthin, phenethyl caffeate, and Curcuma longa L. extract were validated as bioactive chemicals that inhibited DHT production in cultured DU145 cells. In addition, naringenin boosted DHT production in DU145 cells.

conclusionThe method can facilitate the discovery of bioactive chemicals that modulate the DHT production, and four phytochemicals are potential candidates of nutraceuticals to adjust DHT levels in male hormonal dysfunction.

Indexed as

Antineoplastic AgentsPlant ExtractsCell Line, TumorCell SurvivalChromatography, LiquidDihydrotestosteroneDrug DiscoveryFlavanonesHigh-Throughput Screening AssaysHumansMaleMass SpectrometryProstatic NeoplasmsTestosteroneXanthophyllsAntineoplastic AgentsDihydrotestosteroneFlavanonesfucoxanthinnaringeninPlant ExtractsTestosteroneXanthophylls5α-reductase inhibitorDihydrotestosteroneFucoxanthinNaringeninTestosterone

Identifiers

PMID29164416
PMCPMC5813082
OpenAlexW2770833193

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.