ArticleWorld journal of microbiology & biotechnology2017
Establishment of an efficient transformation system for Pleurotus ostreatus.
Article in World journal of microbiology & biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Glutathione peroxidase gene regulates heat stress response via trehalose synthesis in Pleurotus ostreatus.Applied microbiology and biotechnology · 2026Article
- The Transformation and Protein Expression of the Edible MushroomJournal of fungi (Basel, Switzerland) · 2025Article
- Current Advances in the Functional Genes of Edible and Medicinal Fungi: Research Techniques, Functional Analysis, and Prospects.Journal of fungi (Basel, Switzerland) · 2024Review
- Genome-wide analysis of bZIP gene family members in Pleurotus ostreatus, and potential roles of PobZIP3 in development and the heat stress response.Microbial biotechnology · 2024Article
- Contrasting effects of NADPH oxidases on the fungal hyphae growth and immune responses inFrontiers in microbiology · 2024Article
- Salicylic Acid Treatment Alleviates the Heat Stress Response by Reducing the Intracellular ROS Level and Increasing the Cytosolic Trehalose Content in Pleurotus ostreatus.Microbiology spectrum · 2023Article
- A lectin gene is involved in the defense ofFrontiers in microbiology · 2023Article
- Functional Roles ofJournal of fungi (Basel, Switzerland) · 2022Article
- Establishment of an Efficient Polyethylene Glycol (PEG)-Mediated Transformation System inJournal of fungi (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Pleurotus ostreatus is widely cultivated worldwide, but the lack of an efficient transformation system regarding its use restricts its genetic research. The present study developed an improved and efficient Agrobacterium tumefaciens-mediated transformation method in P. ostreatus. Four parameters were optimized to obtain the most efficient transformation method. The strain LBA4404 was the most suitable for the transformation of P. ostreatus. A bacteria-to-protoplast ratio of 100:1, an acetosyringone (AS) concentration of 0.1 mM, and 18 h of co-culture showed the best transformation efficiency. The hygromycin B phosphotransferase gene (HPH) was used as the selective marker, and EGFP was used as the reporter gene in this study. Southern blot analysis combined with EGFP fluorescence assay showed positive results, and mitotic stability assay showed that more than 75% transformants were stable after five generations. These results showed that our transformation method is effective and stable and may facilitate future genetic studies in P. ostreatus.
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Registered trials
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