Evidence map›Paper›PMID 29163448›Full record

ArticleFrontiers in microbiology2017

Real-Time qPCR as a Method for Detection of Antibody-Neutralized Phage Particles.

Anna Kłopot, Adriana Zakrzewska, Dorota Lecion, Joanna M Majewska, Marek A Harhala, Karolina Lahutta, Zuzanna Kaźmierczak, Łukasz Łaczmański, Marlena Kłak, Krystyna Dąbrowska

Open access · goldAbstract read
In one paragraph

Article in Frontiers in microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 19 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Considerations for the Use of Phage Therapy in Clinical Practice.Antimicrobial agents and chemotherapy · 2022
    Review
  6. Review
  7. Roborovski hamsterVirulence · 2021
    Article
  8. Article
  9. Bacteriophage Enumeration and Detection Methods.Frontiers in microbiology · 2020
    Review
  10. Article
  11. Review
  12. Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Anna KłopotBacteriophage Laboratory, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
Adriana ZakrzewskaBacteriophage Laboratory, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
Dorota LecionBacteriophage Laboratory, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
Joanna M MajewskaBacteriophage Laboratory, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
Marek A HarhalaBacteriophage Laboratory, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
Karolina LahuttaBacteriophage Laboratory, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
Zuzanna KaźmierczakBacteriophage Laboratory, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
Łukasz ŁaczmańskiBacteriophage Laboratory, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
Marlena KłakResearch and Development Center, Regional Specialist Hospital, Wrocław, Poland.
Krystyna DąbrowskaBacteriophage Laboratory, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
Polish Academy of Sciences · PLŁukasiewicz Research Network – PORT Polish Center for Technology Development · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The most common method for phage quantitation is the plaque assay, which relies on phage ability to infect bacteria. However, non-infective phage particles may preserve other biological properties; specifically, they may enter interactions with the immune system of animals and humans. Here, we demonstrate real-time quantitative polymerase chain reaction (qPCR) detection of bacteriophages as an alternative to the plaque assay. The closely related staphylococcal bacteriophages A3R and 676Z and the coliphage T4 were used as model phages. They were tested

Indexed as

antibodyhumoral responseimmune responseneutralizingphageplaque assayqPCRquantitation

Identifiers

PMID29163448
PMCPMC5672142
OpenAlexW2770282499

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.