ArticleFrontiers in microbiology2017
MinION Nanopore Sequencing Enables Correlation between Resistome Phenotype and Genotype of Coliform Bacteria in Municipal Sewage.
Article in Frontiers in microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Nanopore Sequencing in Veterinary Pathogen Detection: A Review of Technologies and Applications.Veterinary sciences · 2026Review
- Detection of Biological Molecules Using Nanopore Sensing Techniques.Biomedicines · 2023Review
- Strategies and tools in illumina and nanopore-integrated metagenomic analysis of microbiome data.iMeta · 2023Review
- Article
- Application of MinION Amplicon Sequencing to Buccal Swab Samples for Improving Resolution and Throughput of Rumen Microbiota Analysis.Frontiers in microbiology · 2022Article
- Metagenomic Analysis Reveals Changes in Bacterial Communities and Antibiotic Resistance Genes in an Eye Specialty Hospital and a General Hospital Before and After Wastewater Treatment.Frontiers in microbiology · 2022Article
- Florfenicol and oxazolidone resistance status in livestock farms revealed by short- and long-read metagenomic sequencing.Frontiers in microbiology · 2022Article
- A review of the resistome within the digestive tract of livestock.Journal of animal science and biotechnology · 2021Review
- Nanopore-based metagenomics analysis reveals prevalence of mobile antibiotic and heavy metal resistome in wastewater.Ecotoxicology (London, England) · 2021Article
- Revealing antimicrobial resistance in stormwater with MinION.Chemosphere · 2020Article
- Experimental approaches to tracking mobile genetic elements in microbial communities.FEMS microbiology reviews · 2020Review
- Two Cases of Vancomycin-ResistantOpen forum infectious diseases · 2020Article
- Rapid MinION profiling of preterm microbiota and antimicrobial-resistant pathogens.Nature microbiology · 2020Article
- Shotgun metagenome data of a defined mock community using Oxford Nanopore, PacBio and Illumina technologies.Scientific data · 2019Article
- Nanopore metagenomics enables rapid clinical diagnosis of bacterial lower respiratory infection.Nature biotechnology · 2019Article
- Mobile antibiotic resistome in wastewater treatment plants revealed by Nanopore metagenomic sequencing.Microbiome · 2019Article
- Applying Rapid Whole-Genome Sequencing To Predict Phenotypic Antimicrobial Susceptibility Testing Results among Carbapenem-Resistant Klebsiella pneumoniae Clinical Isolates.Antimicrobial agents and chemotherapy · 2019Article
- Diverse Fluoroquinolone Resistance Plasmids From Retail MeatFrontiers in microbiology · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wastewater treatment plants (WWTPs) functioned as the intersection between the human society and nature environment, are receiving increasingly more attention on risk assessment of the acquisition of environmental antibiotic resistance genes (ARGs) by pathogenetic populations during treatment. However, because of the general lack of robust resistome profiling methods, genotype, and resistance phenotype is still poorly correlated in human pathogens of sewage samples. Here we applied MinION sequencing to quantify the resistance genes of multiple antibiotic resistant (MAR) coliform bacteria, a common indicator for human enteric pathogens in sewage samples. Our pipeline could deliver the results within 30 h from sample collection and the resistome quantification was consistent to that based on the Illumina platform. Additionally, the long nanopore reads not only enabled a simultaneous identification of the carrier populations of ARGs detected, but also facilitated the genome reconstruction of a representative MAR strain, from which we identified an instance of chromosomal integration of environmental resistance gene obtained by plasmid exchange with a porcine pathogen. This study demonstrated the utilization of MinION sequencing in quick monitoring and simultaneous phylogenetic tracking of environmental ARGs to address potential health risk associated with them.
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