ArticleFrontiers in pharmacology2017
A Dual Role of P53 in Regulating Colistin-Induced Autophagy in PC-12 Cells.
Article in Frontiers in pharmacology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Colistin Induces Oxidative Stress and Apoptotic Cell Death through the Activation of the AhR/CYP1A1 Pathway in PC12 Cells.Antioxidants (Basel, Switzerland) · 2024Article
- Autocrine role of senescent cardiac fibroblasts-derived extracellular vesicles.The Journal of veterinary medical science · 2023Article
- Isolation of Fucoxanthin fromBiomedicines · 2023Article
- Population Pharmacokinetics of Polymyxin B and Dosage Optimization in Renal Transplant Patients.Frontiers in pharmacology · 2021Article
- Antiproliferative effects of L-asparaginase in acute myeloid leukemia.Experimental and therapeutic medicine · 2020Article
- Polymyxins-Curcumin Combination Antimicrobial Therapy: Safety Implications and Efficacy for Infection Treatment.Antioxidants (Basel, Switzerland) · 2020Review
- Autophagy and its regulation by ginseng components.Journal of ginseng research · 2019Review
- The molecular basis for p53 inhibition of autophagy in porcine fibroblast cells.Translational cancer research · 2019Article
- Baicalin AttenuatesInfection and drug resistance · 2019Article
- Immunomodulatory Effects of Colistin on Macrophages in Rats by Activating the p38/MAPK Pathway.Frontiers in pharmacology · 2019Article
- LINC00470 Coordinates the Epigenetic Regulation of ELFN2 to Distract GBM Cell Autophagy.Molecular therapy : the journal of the American Society of Gene Therapy · 2018Article
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study aimed to investigate the mechanism of p53 in regulating colistin-induced autophagy in PC-12 cells. Importantly, cells were treated with 125 μg/ml colistin for 12 and 24 h after transfection with p53 siRNA or recombinant plasmid. The hallmarks of autophagy and apoptosis were examined by real-time PCR and western blot, fluorescence/immunofluorescence microscopy, and electron microscopy. The results showed that silencing of p53 leads to down-regulation of Atg5 and beclin1 for 12 h while up-regulation at 24 h and up-regulation of p62 noted. The ratio of LC3-II/I and autophagic vacuoles were significantly increased at 24 h, but autophagy flux was blocked. The cleavage of caspase3 and PARP (poly ADP-ribose polymerase) were enhanced, while PC-12-sip53 cells exposed to 3-MA showed down-regulation of apoptosis. By contrast, the expression of autophagy-related genes and protein reduced in p53 overexpressing cells following a time dependent manner. Meanwhile, there was an increase in the expression of activated caspase3 and PARP, condensed and fragmented nuclei were evident. Conclusively, the data supported that silencing of p53 promotes impaired autophagy, which acts as a pro-apoptotic induction factor in PC-12 cells treated with colistin for 24 h, and overexpression of p53 inhibits autophagy and accelerates apoptosis. Hence, it has been suggested that p53 could not act as a neuro-protective target in colistin-induced neurotoxicity.
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