ArticleScientific reports2017
AFM-based detection of glycocalyx degradation and endothelial stiffening in the db/db mouse model of diabetes.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Beneficial vascular effects of oral phosphatidylserine supplementation in type 2 diabetes.Journal of applied physiology (Bethesda, Md. : 1985) · 2026Trial
- Impact of dietary supplementation of glycocalyx precursors on vascular function in type 2 diabetes.Journal of applied physiology (Bethesda, Md. : 1985) · 2024Trial
- Mineralocorticoid receptor antagonism in diabetes reduces albuminuria by preserving the glomerular endothelial glycocalyx.JCI insight · 2023Trial
- Heparanase, Endothelial Dysfunction, and Cardiovascular Risk: The Emerging Role of Vitamin D.ACS pharmacology & translational science · 2026Review
- Guidelines for evaluating endothelial function in vascular tissue.American journal of physiology. Heart and circulatory physiology · 2026Review
- Manuscript DMD-D-25-00346 The impact of glycocalyx on partitioning and distribution of basic drugs.Drug metabolism and disposition: the biological fate of chemicals · 2026Article
- Integrated transcriptome and proteome analyses unveil cytoskeletal alterations in an endothelial model of monogenic diabetes.Genome medicine · 2026Article
- Sex differences in arterial stiffness in a rat model of type 1 diabetes.Frontiers in physiology · 2026Article
- Finite Element Analysis of a PTFE Felt Interposition Pad for Relieving Vascular Compression of the Trigeminal Nerve.Applied bionics and biomechanics · 2026Article
- Nanomechanical Characterization of E-Cigarette-Induced Lung Endothelial Dysfunction: Roles of Cortactin and Mitochondrial Reactive Oxygen Species.International journal of molecular sciences · 2025Article
- Inhibition of lipolysis in visceral adipose tissue from obese mice and humans prevents impairment of endothelial Kir2.1 channels.Channels (Austin, Tex.) · 2025Article
- Mechanical Properties of Endothelial Cells: A Key to Physiology, Drug Testing and Nanostructure Interaction.Cells · 2025Review
- Liver Sinusoidal Endothelial Cells and Their Regulation of Immunology, Collagenization, and Bioreactivity in Fatty Liver: A Narrative Review.International journal of molecular sciences · 2025Review
- Reduced cofilin activity as a mechanism contributing to endothelial cell stiffening in type 2 diabetes.American journal of physiology. Heart and circulatory physiology · 2025Article
- Endothelial glycocalyx in different flow regions of the trabecular outflow pathway in bovine eyes.Frontiers in cell and developmental biology · 2025Article
- Detection of Human Bladder Epithelial Cancerous Cells with Atomic Force Microscopy and Machine Learning.Cells · 2024Article
- Probing living cell dynamics and molecular interactions using atomic force microscopy.Biophysical reviews · 2024Review
- Visceral adipose of obese mice inhibits endothelial inwardly rectifying KAmerican journal of physiology. Cell physiology · 2024Article
- Review
- Neuraminidase inhibition improves endothelial function in diabetic mice.American journal of physiology. Heart and circulatory physiology · 2023Article
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Authors and funding
9 authors.
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Abstract
Degradation of the glycocalyx and stiffening of endothelium are important pathophysiological components of endothelial dysfunction. However, to our knowledge, these events have not been investigated in tandem in experimental diabetes. Here, the mechanical properties of the glycocalyx and endothelium in ex vivo mouse aorta were determined simultaneously in indentation experiments with an atomic force microscope (AFM) for diabetic db/db and control db/+ mice at ages of 11-19 weeks. To analyze highly heterogeneous aorta samples, we developed a tailored classification procedure of indentation data based on a bi-layer brush model supplemented with Hertz model for quantification of nanomechanics of endothelial regions with and without the glycocalyx surface. In db/db mice, marked endothelial stiffening and reduced glycocalyx coverage were present already in 11-week-old mice and persisted in older animals. In contrast, reduction of the effective glycocalyx length was progressive and was most pronounced in 19-week-old db/db mice. The reduction of the glycocalyx length correlated with an increasing level of glycated haemoglobin and decreased endothelial NO production. In conclusion, AFM nanoindentation analysis revealed that stiffening of endothelial cells and diminished glycocalyx coverage occurred in early diabetes and were followed by the reduction of the glycocalyx length that correlated with diabetes progression.
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