Evidence map›Paper›PMID 29162149›Full record

ArticleBMC research notes2017

High-throughput SuperSAGE for gene expression analysis of Nicotiana tabacum-Rhizoctonia solani interaction.

Roxana Portieles, María Elena Ochagavia, Eduardo Canales, Yussuan Silva, Osmani Chacón, Ingrid Hernández, Yunior López, Mayra Rodríguez, Ryohei Terauchi, Carlos Borroto and 4 more

Open access · goldAbstract read
In one paragraph

Article in BMC research notes, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 8 institutions in 6 countries.

Roxana PortielesCenter for Genetic Engineering and Biotechnology, 10600, Havana, Cuba.
María Elena OchagaviaCenter for Genetic Engineering and Biotechnology, 10600, Havana, Cuba.
Eduardo CanalesCenter for Genetic Engineering and Biotechnology, 10600, Havana, Cuba.
Yussuan SilvaTobacco Research Institute, Carretera de Tumbadero 8, 6063, San Antonio de los Baños, Havana, Cuba.
Osmani ChacónTobacco Research Institute, Carretera de Tumbadero 8, 6063, San Antonio de los Baños, Havana, Cuba.
Ingrid HernándezCenter for Genetic Engineering and Biotechnology, 10600, Havana, Cuba.
Yunior LópezCenter for Genetic Engineering and Biotechnology, 10600, Havana, Cuba.
Mayra RodríguezCenter for Genetic Engineering and Biotechnology, 10600, Havana, Cuba.
Ryohei TerauchiIwate Biotechnology Research Center, Kitakami, Iwate, 024-0003, Japan.
Carlos BorrotoCentro de Investigación Científica de Yucatán, Calle 43 No. 130, Colonia Chuburná de Hidalgo, 97200, Mérida, Yucatán, Mexico.
Ramón SantosUniversidad Técnica Luis Vargas Torres de Esmeraldas, Av. Kennedy 704, Esmeraldas, Ecuador.
Melvin D BoltonUSDA-Agricultural Research Service, Northern Crops Science Laboratory, 1605 Albrecht Blvd., Fargo, ND, 58102-2765, USA.
Camilo Ayra-PardoCenter for Genetic Engineering and Biotechnology, 10600, Havana, Cuba.
Orlando Borrás-HidalgoCenter for Genetic Engineering and Biotechnology, 10600, Havana, Cuba. borrasorlando@yahoo.com.
Centro de Ingeniería Genética y Biotecnología · CUTobacco Research Institute · CNEdward T. Schafer Agricultural Research Center · USIwate Biotechnology Research Center · JPNanyang Normal University · CNQilu University of Technology · CNSecretaría de Investigación, Innovación y Educación Superior · MXUniversidad Técnica Luis Vargas Torres · EC

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThe ubiquitous soil pathogen Rhizoctonia solani causes serious diseases in different plant species. Despite the importance of this disease, little is known regarding the molecular basis of susceptibility. SuperSAGE technology and next-generation sequencing were used to generate transcript libraries during the compatible Nicotiana tabacum-R. solani interaction. Also, we used the post-transcriptional silencing to evaluate the function of a group of important genes.

resultsA total of 8960 and 8221 unique Tag sequences identified as differentially up- and down-regulated were obtained. Based on gene ontology classification, several annotated UniTags corresponded to defense response, metabolism and signal transduction. Analysis of the N. tabacum transcriptome during infection identified regulatory genes implicated in a number of hormone pathways. Silencing of an mRNA induced by salicylic acid reduced the susceptibility of N. tabacum to R. solani. We provide evidence that the salicylic acid pathway was involved in disease development. This is important for further development of disease management strategies caused by this pathogen.

Indexed as

Gene Expression ProfilingExpressed Sequence TagsGenes, PlantHigh-Throughput Nucleotide SequencingNicotianaRhizoctoniaRNA InterferenceRhizoctonia solaniSalicylic acidSuperSAGETobacco

Identifiers

PMID29162149
PMCPMC5697063
OpenAlexW2770700201

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.