Evidence map›Paper›PMID 29162131›Full record

ArticleBMC research notes2017

Absence of nuclease activity in commonly used oxygen-scavenging systems.

Hailey L Gahlon, Paul Poudevigne-Durance, David Rueda

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC research notes, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 89% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Hailey L GahlonMolecular Virology, Department of Medicine, Imperial College London, Du Cane Road, London, W12 0NN, UK.
Paul Poudevigne-DuranceMolecular Virology, Department of Medicine, Imperial College London, Du Cane Road, London, W12 0NN, UK.
David RuedaMolecular Virology, Department of Medicine, Imperial College London, Du Cane Road, London, W12 0NN, UK. david.rueda@imperial.ac.uk.ORCID http://orcid.org/0000-0003-4657-6323
MRC London Institute of Medical Sciences · GB

Funding

H2020 Marie Skłodowska-Curie Actions (BE) 705015Medical Research Council MC_UP_1102/5Medical Research Council (GB) MC-A658-5TY10
6 · The paper itself

Abstract

objectiveOxygen scavenging systems are routinely used during single-molecule imaging experiments to improve fluorescent dye stability. Previous work has shown nuclease contamination in the commonly used oxygen scavenging systems. This study evaluates the potential for nuclease contamination in these oxygen scavenging systems.

resultsLinear and plasmid DNA was incubated with two different oxygen scavenging systems (1) protocatechuic acid (PCA)-protocatechuate-3,4-dioxygenase (PCD) and (2) glucose-coupled glucose oxidase/catalase (GODCAT). No nucleic acid degradation was observed on single and double-stranded linear DNA and plasmid DNA, indicating the absence of nuclease contamination in these oxygen scavenging systems.

Indexed as

CatalaseChromatography, GelDeoxyribonucleasesDNAGlucose OxidaseHydroxybenzoatesIndicators and ReagentsOxygenPlasmidsProtocatechuate-3,4-DioxygenaseReactive Oxygen SpeciesCatalaseDeoxyribonucleasesDNAGlucose OxidaseHydroxybenzoatesIndicators and ReagentsOxygenProtocatechuate-3,4-Dioxygenaseprotocatechuic acidReactive Oxygen SpeciesCatalaseGlucose oxidaseOxygen scavenging systemProtocatechuate-3,4-dioxygenaseProtocatechuic acidSingle-molecule imaging

Identifiers

PMID29162131
PMCPMC5697020
OpenAlexW2769456841

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.