ArticlePLoS biology2017
Nucleic acid purification from plants, animals and microbes in under 30 seconds.
Article in PLoS biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 110 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
110 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Detection of Viral Nucleic Acid in Specimens Spotted on Commercial Filter Papers: A Review and Meta-Analysis.Viruses · 2026Pooled it
- Colourimetric LAMP detection of Escherichia coli in bovine milk using a simplified DNA preparation method.Veterinary research communications · 2026Article
- Detection ofAnimals : an open access journal from MDPI · 2026Article
- Revolutionizing Healthcare With Paper-Based Nucleic Acid Testing.Exploration (Beijing, China) · 2026Review
- Rapid On-Site Detection ofPlants (Basel, Switzerland) · 2026Article
- Intelligent microfluidic device for multiplex detection and prompt warning of upper and lower respiratory tract infections.Journal of advanced research · 2026Article
- Trends of nucleic acid - based point-of-care diagnostics for infectious diseases.Journal of biological engineering · 2026Review
- Research progress on nucleic acid amplification-based detection technologies for phytopathogenic fungi.Applied microbiology and biotechnology · 2026Review
- A Field-Deployable eDNA Metabarcoding Workflow Including De Novo Reference Assembly for Characterising Understudied Biodiversity Hotspots.Molecular ecology resources · 2026Article
- Variability analysis of a low-cost paper dipstick nucleic acid extraction method for wastewater surveillance using gage repeatability and reproducibility.Scientific reports · 2026Article
- Electroelution Into a Salt Trap: Reviving an Old-School Approach to DNA Purification.Small methods · 2026Article
- Revisions to theMycoKeys · 2026Article
- Material-to-Application Integration: Rapid Fabrication of Field-Deployable Hydrogel-SiOAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Development of a novel Cas13a/Cas12a-mediated 'one-pot' dual detection assay for genetically modified crops.Journal of advanced research · 2025Article
- Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Sexually Transmitted Infections: A Review.Microbial biotechnology · 2025Review
- Rapid tuberculosis diagnosis from respiratory or blood samples by a low cost, portable lab-in-tube assay.Science translational medicine · 2025Article
- Optimized Protocol for High-Quality RNA Extraction from Grape Berry Skins Using Sorbitol Pre-Wash.Plants (Basel, Switzerland) · 2025Article
- A Simplified, CRISPR-Based Method for the Detection of Batrachochytrium salamandrivorans.EcoHealth · 2025Article
- Super-Fast Detection ofFoods (Basel, Switzerland) · 2025Article
- Comparison of the recovery of PCR-detectable porcine epidemic diarrhea virus and porcine reproductive and respiratory syndrome virus from filter papers under laboratory conditions.Frontiers in cellular and infection microbiology · 2025Article
50 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleic acid amplification is a powerful molecular biology tool, although its use outside the modern laboratory environment is limited due to the relatively cumbersome methods required to extract nucleic acids from biological samples. To address this issue, we investigated a variety of materials for their suitability for nucleic acid capture and purification. We report here that untreated cellulose-based paper can rapidly capture nucleic acids within seconds and retain them during a single washing step, while contaminants present in complex biological samples are quickly removed. Building on this knowledge, we have successfully created an equipment-free nucleic acid extraction dipstick methodology that can obtain amplification-ready DNA and RNA from plants, animals, and microbes from difficult biological samples such as blood and leaves from adult trees in less than 30 seconds. The simplicity and speed of this method as well as the low cost and availability of suitable materials (e.g., common paper towelling), means that nucleic acid extraction is now more accessible and affordable for researchers and the broader community. Furthermore, when combined with recent advancements in isothermal amplification and naked eye DNA visualization techniques, the dipstick extraction technology makes performing molecular diagnostic assays achievable in limited resource settings including university and high school classrooms, field-based environments, and developing countries.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.