Evidence map›Paper›PMID 29159972›Full record

ArticleJournal of cachexia, sarcopenia and muscle2018

Vitamin B12 deficiency and impaired expression of amnionless during aging.

Alice Pannérec, Eugenia Migliavacca, Antonio De Castro, Joris Michaud, Sonia Karaz, Laurence Goulet, Serge Rezzi, Tze Pin Ng, Nabil Bosco, Anis Larbi and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cachexia, sarcopenia and muscle, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
3.5field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 50 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Article
  6. Article
  7. Vitamin BInternational journal of molecular sciences · 2024
    Review
  8. Article
  9. Emerging Roles of Vitamin BInternational journal of molecular sciences · 2024
    Review
  10. Article
  11. Article
  12. Article
  13. Review
  14. B12 levels and frailty syndrome.Journal of frailty, sarcopenia and falls · 2022
    Review
  15. Article
  16. Article
  17. Review
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 2 countries.

Alice PannérecNestlé Institute of Health Sciences, EPFL Innovation Park, Building H, 1015, Lausanne, Switzerland.
Eugenia MigliavaccaNestlé Institute of Health Sciences, EPFL Innovation Park, Building H, 1015, Lausanne, Switzerland.
Antonio De CastroNestlé Research Centre, Singapore.
Joris MichaudNestlé Institute of Health Sciences, EPFL Innovation Park, Building H, 1015, Lausanne, Switzerland.
Sonia KarazNestlé Institute of Health Sciences, EPFL Innovation Park, Building H, 1015, Lausanne, Switzerland.
Laurence GouletNestlé Institute of Health Sciences, EPFL Innovation Park, Building H, 1015, Lausanne, Switzerland.
Serge RezziNestlé Institute of Health Sciences, EPFL Innovation Park, Building H, 1015, Lausanne, Switzerland.
Tze Pin NgGerontology Research Programme, Department of Psychological Medicine, Young Loo Lin School of Medicine, National University of Singapore, Singapore.
Nabil BoscoNestlé Research Centre, Singapore.
Anis LarbiSingapore Immunology Network, Biopolis, Agency for Science, Technology and Research, Singapore.
Jerome N FeigeNestlé Institute of Health Sciences, EPFL Innovation Park, Building H, 1015, Lausanne, Switzerland.
École Polytechnique Fédérale de Lausanne · CHNestlé (Singapore) · SGMinistry of Health · SGNestlé (Switzerland) · CHSingapore Immunology Network · SG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPhysical frailty and loss of mobility in elderly individuals lead to reduced independence, quality of life, and increased mortality. Vitamin B12 deficiency has been linked to several age-related chronic diseases, including in the musculo-skeletal system, where vitamin B12 deficiency is generally believed to be linked to poor nutritional intake. In the present study, we asked whether aging and frailty associate with altered vitamin B12 homeostasis in humans and investigated the underlying molecular mechanisms using preclinical models.

methodsWe analysed a subset of the Singapore Longitudinal Aging Study and stratified 238 participants based on age and Fried frailty criteria. Levels of methyl-malonic acid (MMA), a marker for vitamin B12 deficiency, and amnionless, the vitamin B12 co-receptor that anchors the vitamin B12 transport complex to the membrane of epithelial cells, were measured in plasma. In addition, vitamin B12 levels and the molecular mechanisms of vitamin B12 uptake and excretion were analysed in ileum, kidney, liver, and blood using a rat model of natural aging where nutritional intake is fully controlled.

resultsWe demonstrate that aging and frailty are associated with a higher prevalence of functional vitamin B12 deficiency that can be detected by increased levels of MMA in blood (ρ = 0.25; P = 0.00013). The decline in circulating vitamin B12 levels is recapitulated in a rat model of natural aging where food composition and intake are stable. At the molecular level, these perturbations involve altered expression of amnionless in the ileum and kidney. Interestingly, we demonstrate that amnionless can be detected in serum where its levels increase during aging in both rodents and human (P = 3.3e-07 and 9.2e-07, respectively). Blood amnionless levels negatively correlate with vitamin B12 in rats (r

conclusionsOur results demonstrate that aging and frailty cause intrinsic vitamin B12 deficiencies, which can occur independently of nutritional intake. Mechanistically, vitamin B12 deficiency involves the physio-pathological decline of both the intestinal uptake and the renal reabsorption system for vitamin B12. Finally, amnionless is a novel biomarker which can detect perturbed vitamin B12 bioavailability during aging and physical frailty.

Indexed as

AdultAgedAged, 80 and overAgingAnimalsBiomarkersFemaleHumansLongitudinal StudiesMaleMembrane ProteinsMethylmalonic AcidMiddle AgedProteinsRatsRats, WistarAMN protein, humanBiomarkersMembrane ProteinsMethylmalonic AcidProteinsVitamin B 12AgingAmnionlessCobalaminFrailtyMethylmalonic acidSarcopeniaVitamin B12

Identifiers

PMID29159972
PMCPMC5803611
OpenAlexW2769439043

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.