ArticleCell proliferation2018
Proliferation and differentiation of mesenchymal stem cells on scaffolds containing chitosan, calcium polyphosphate and pigeonite for bone tissue engineering.
Article in Cell proliferation, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed.
- Sequential Regulation of Neurovascular Bone Regeneration in Large Bone Defects Using Black Phosphorus-Europium Nanosheet Integrated TPMS Biomimetic Scaffold with Photo-Controlled Ion Release.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Polyphosphate in Bone Tissue Engineering: From Molecular Mechanisms to Material Design.Journal of functional biomaterials · 2026Review
- Dental pulp stem cells and hydroxyapatite: a promising combination for maxillary bone regeneration.BMC oral health · 2026Article
- Review
- A multi-stage weakly supervised design for spheroid segmentation to explore mesenchymal stem cell differentiation dynamics.BMC bioinformatics · 2025Article
- Regeneration of Rabbit Calvarial Defects with Combination of Stem Cells and Enamel Matrix Derivative: A Microcomputed Tomography and Histological Evaluation Comparing Two- and Three-Dimensional Cell Constructs.Medicina (Kaunas, Lithuania) · 2024Article
- [Advances in Molecular Regulatory Mechanisms of Jaw Repair and Reconstruction].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2024Review
- Article
- Discussing the final size and shape of the reconstructed tissues in tissue engineering.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2023Review
- Progress and emerging techniques for biomaterial-based derivation of mesenchymal stem cells (MSCs) from pluripotent stem cells (PSCs).Biomaterials research · 2023Review
- Review
- Calcium-Polyphosphate Submicroparticles (CaPP) Improvement Effect of the Experimental Bleaching Gels' Chemical and Cellular-Viability Properties.Gels (Basel, Switzerland) · 2023Article
- Effects of erythropoietin on osteoblast in the tooth extraction socket in mice periodontitis model.Frontiers in physiology · 2022Article
- Fluorapatite and fluorohydroxyapatite apatite surfaces drive adipose-derived stem cells to an osteogenic lineage.Journal of the mechanical behavior of biomedical materials · 2022Article
- The polysaccharide chitosan facilitates the isolation of small extracellular vesicles from multiple biofluids.Journal of extracellular vesicles · 2021Article
- Strategies for Bone Regeneration: From Graft to Tissue Engineering.International journal of molecular sciences · 2021Review
- Interleukin-4-loaded hydrogel scaffold regulates macrophages polarization to promote bone mesenchymal stem cells osteogenic differentiation via TGF-β1/Smad pathway for repair of bone defect.Cell proliferation · 2020Article
- Nanocomposite Films of Chitosan-Grafted Carbon Nano-Onions for Biomedical Applications.Molecules (Basel, Switzerland) · 2020Article
- Adipose-Derived Mesenchymal Stem Cell Chondrospheroids Cultured in Hypoxia and a 3D Porous Chitosan/Chitin Nanocrystal Scaffold as a Platform for Cartilage Tissue Engineering.International journal of molecular sciences · 2020Article
- Lanthanides-Substituted Hydroxyapatite/International journal of nanomedicine · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesTreatment of critical-sized bone defects with cells and biomaterials offers an efficient alternative to traditional bone grafts. Chitosan (CS) is a natural biopolymer that acts as a scaffold in bone tissue engineering (BTE). Polyphosphate (PolyP), recently identified as an inorganic polymer, acts as a potential bone morphogenetic material, whereas pigeonite (Pg) is a novel iron-containing ceramic. In this study, we prepared and characterized scaffolds containing CS, calcium polyphosphate (CaPP) and Pg particles for bone formation in vitro and in vivo. MATERIALS AND
methodsChitosan/CaPP scaffolds and CS/CaPP scaffolds containing varied concentrations of Pg particles (0.25%, 0.5%, 0.75% and 1%) were prepared and characterized by SEM, XRD, EDAX, FT-IR, degradation, protein adsorption, mechanical strength and biomineralization studies. The cytocompatibility of these scaffolds with mouse mesenchymal stem cells (mMSCs, C3H10T1/2) was determined by MTT assay and fluorescence staining. Cell proliferation on scaffolds was assessed using MUSE
resultsThe inclusion of iron-containing Pg particles at 0.25% concentration in CS/CaPP scaffolds showed enhanced bioactivity by protein adsorption and biomineralization, compared with that shown by CS/CaPP scaffolds alone. Increased proliferation of mMSCs was observed with CS/CaPP/Pg scaffolds compared with control and CS/CaPP scaffolds. Increase in cell proliferation was accompanied by G0/G1 to G2/M phase transition with increased levels of cyclin(s) A, B and C. Pg particles in CS/CaPP scaffolds enhanced osteoblast differentiation at the cellular and molecular levels, as evidenced by increased calcium deposits, ALP activity and expression of osteoblast marker genes. In vivo implantation of scaffolds in rat critical-sized tibial defects displayed accelerated bone formation after 8 weeks.
conclusionThe current findings indicate that CS/CaPP scaffolds containing iron-containing Pg particles serve as an appropriate template to support proliferation and differentiation of MSCs to osteoblasts in vitro and bone formation in vivo and thus support their candidature for BTE applications.
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