ArticlePractical laboratory medicine2017
Cirrhosis of liver: Interference of serpins in quantification of SERPINA4 - A preliminary study.
Article in Practical laboratory medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 17 citations in OpenAlex.
- Grass carp SERPINA1 inhibits GCRV infection through degrading CF2.Frontiers in immunology · 2022Article
- Early-life undernutrition induces enhancer RNA remodeling in mice liver.Epigenetics & chromatin · 2021Article
- Identification and characterization of sexual dimorphism‑linked gene expression profile in hepatocellular carcinoma.Oncology reports · 2019Article
- Article
- Evolution of proteomic biomarker for chronic liver disease: Promise into reality.Journal of circulating biomarkersReview
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCirrhosis of liver is a pathological condition, wherein functions of liver are impaired by chronic liver exploitations. Due to decrease in synthetic capacity, expressions of plasma proteins tend to decrease in blood stream. Serpins (Serine protease inhibitors) are class of plasma proteins expressed from liver with structural similarities and diverse functions. SERPINA4 (Kallistatin) is a multifunctional serpin clade A protein expressed from liver and concentration in serum is the reflection of extent of liver dysfunction.
objectiveTo identify interference of other serpins by immunological cross reactivity with SERPINA4 in cirrhotic liver and healthy subjects. MATERIALS AND
methodsBlood samples were collected from 20 subjects (10 cirrhotic liver, 10 healthy) from R.L. Jalappa Hospital and Research Centre, Kolar, Karnataka, India. Separation of proteins was carried out by SDS-PAGE. Cross reactivity study was analyzed using western blot.
resultsProteins present in cirrhotic liver and healthy subject's serum were separated by SDS PAGE. There was no band detection on both (cirrhotic liver and healthy) PVDF (polyvinylidene diflouride) membranes. However, a significant band was observed with recombinant kallistatin.
conclusionStructurally similar serpins with minor amino acid sequence similarities did not show any immunological cross reactivity with SERPINA4 due to non identical epitope in cirrhotic liver and healthy subjects. Present study revealed that there is no interference of serpins for immunological reactions in quantitative estimation of kallistatin which needs further validation.
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