ArticleNature communications2017
Programmable DNA looping using engineered bivalent dCas9 complexes.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- 3D Genome Engineering Using CRISPR/dCas Systems.International journal of molecular sciences · 2026Review
- Engineering the Link: From Genome Interaction Maps to Functional Insight.Advanced biology · 2025Review
- Profiling crRNA architectures for enhanced Cas12 biosensing.Communications biology · 2025Article
- 3D Genome Engineering: Current Advances and Therapeutic Opportunities in Human Diseases.Research (Washington, D.C.) · 2025Review
- DNA regulatory element cooperation and competition in transcription.BMB reports · 2024Review
- Next-generation CRISPR technology for genome, epigenome and mitochondrial editing.Transgenic research · 2024Review
- Engineered transcription activator-like effector dimer proteins confer DNA loop-dependent gene repression comparable to Lac repressor.Nucleic acids research · 2024Article
- Re-engineered guide RNA enables DNA loops and contacts modulating repression in E. coli.Nucleic acids research · 2024Article
- Loops are geometric catalysts for DNA integration.Nucleic acids research · 2024Article
- The Blueprint of Logical Decisions in a NF-κB Signaling System.ACS omega · 2024Article
- Interplay between epigenome and 3D chromatin structure.BMB reports · 2023Review
- Long-range gene regulation in hormone-dependent cancer.Nature reviews. Cancer · 2023Review
- CRISPR-Based Gene Editing inPharmaceuticals (Basel, Switzerland) · 2023Review
- 3D spatial genome organization in the nervous system: From development and plasticity to disease.Neuron · 2022Review
- Manipulating complex chromatin folding via CRISPR-guided bioorthogonal chemistry.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Coarse-grained modeling reveals the impact of supercoiling and loop length in DNA looping kinetics.Biophysical journal · 2022Article
- Epigenome rewiring in human pluripotent stem cells.Trends in cell biology · 2022Review
- Chemically Induced Chromosomal Interaction (CICI) method to study chromosome dynamics and its biological roles.Nature communications · 2022Article
- A chromosomal loop anchor mediates bacterial genome organization.Nature genetics · 2022Article
- Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA looping is a ubiquitous and critical feature of gene regulation. Although DNA looping can be efficiently detected, tools to readily manipulate DNA looping are limited. Here we develop CRISPR-based DNA looping reagents for creation of programmable DNA loops. Cleavage-defective Cas9 proteins of different specificity are linked by heterodimerization or translational fusion to create bivalent complexes able to link two separate DNA regions. After model-directed optimization, the reagents are validated using a quantitative DNA looping assay in E. coli. Looping efficiency is ~15% for a 4.7 kb loop, but is significantly improved by loop multiplexing with additional guides. Bivalent dCas9 complexes are also used to activate endogenous norVW genes by rewiring chromosomal DNA to bring distal enhancer elements to the gene promoters. Such reagents should allow manipulation of DNA looping in a variety of cell types, aiding understanding of endogenous loops and enabling creation of new regulatory connections.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.