Evidence map›Paper›PMID 29157305›Full record

ArticleBMC research notes2017

Evaluation of the effect of a floxed Neo cassette within the dystroglycan (Dag1) gene.

Francesca Sciandra, Bianca Maria Scicchitano, Giulia Signorino, Maria Giulia Bigotti, Barbara Tavazzi, Francesca Lombardi, Manuela Bozzi, Gigliola Sica, Bruno Giardina, Sandra Blaess and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC research notes, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 3 countries.

Francesca SciandraIstituto di Chimica del Riconoscimento Molecolare (CNR), c/o Università Cattolica del Sacro Cuore, Rome, Italy. francesca.sciandra@icrm.cnr.it.ORCID http://orcid.org/0000-0002-2123-1516
Bianca Maria ScicchitanoIstituto di Istologia, Università Cattolica del Sacro Cuore, Rome, Italy.
Giulia SignorinoIstituto di Biochimica e Biochimica Clinica, Università Cattolica del Sacro Cuore, Rome, Italy.
Maria Giulia BigottiSchool of Biochemistry, University of Bristol, Bristol, BS8 1TD, UK.
Barbara TavazziIstituto di Biochimica e Biochimica Clinica, Università Cattolica del Sacro Cuore, Rome, Italy.
Francesca LombardiIstituto di Clinica delle Malattie Infettive, Università Cattolica del Sacro Cuore, Rome, Italy.
Manuela BozziIstituto di Biochimica e Biochimica Clinica, Università Cattolica del Sacro Cuore, Rome, Italy.
Gigliola SicaIstituto di Istologia, Università Cattolica del Sacro Cuore, Rome, Italy.
Bruno GiardinaIstituto di Biochimica e Biochimica Clinica, Università Cattolica del Sacro Cuore, Rome, Italy.
Sandra BlaessNeurodevelopmental Genetics, Institute of Reconstructive Neurobiology, Life and Brain Center, University of Bonn, Bonn, Germany.
Andrea BrancaccioIstituto di Chimica del Riconoscimento Molecolare (CNR), c/o Università Cattolica del Sacro Cuore, Rome, Italy. andrea.brancaccio@icrm.cnr.it.
Università Cattolica del Sacro Cuore · ITUniversity of Bonn · DEUniversity of Bristol · GB

Funding

PRIN 20109MXHMR_001Wellcome Trust
6 · The paper itself

Abstract

objectiveDystroglycan (DG) is an adhesion complex formed by two subunits, α-DG and β-DG. In skeletal muscle, DG is part of the dystrophin-glycoprotein complex that is crucial for sarcolemma stability and it is involved in a plethora of muscular dystrophy phenotypes. Due to the important role played by DG in skeletal muscle stability as well as in a wide variety of other tissues including brain and the peripheral nervous system, it is essential to investigate its genetic assembly and transcriptional regulation.

resultsHerein, we analyze the effect of the insertion of a floxed neomycin (Neo) cassette within the 3' portion of the universally conserved IG1-intron of the DG gene (Dag1). We analyzed the transcription level of Dag1 and the expression of the DG protein in skeletal muscle of targeted mice compared to wild-type and we did not find any alterations that might be attributed to the gene targeting. However, we found an increase of the cross-sectional areas of tibialis anterior that might have some physiological significance that needs to be assessed in the future. Moreover, in targeted mice the skeletal muscle morphology and its regeneration capacity after injury did not show any evident alterations. We confirmed that the targeting of Dag1 with a floxed Neo-cassette did not produce any gross undesired effects.

Indexed as

Mutagenesis, InsertionalNeomycinAnimalsConserved SequenceDystroglycansFemaleGene ExpressionIntegrasesMaleMiceMice, Inbred C57BLMuscle, SkeletalCre recombinaseDag1 protein, mouseDystroglycansIntegrasesNeomycinDystroglycanIncomplete recombinationSkeletal muscle

Identifiers

PMID29157305
PMCPMC5696793
OpenAlexW2769260467

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.